Identification of Virulence Genes and Multidrug Resistance in Shiga-Toxin Producing Escherichia coli (STEC) from Migratory and Captive Wild Birds

被引：5

作者：

Rahman, Asikur ^{[1
]}

Chowdhury, Md. Shahidur Rahman ^{[1
]}

Hossain, Hemayet ^{[2
]}

Elsaid, Fahmy Gad ^{[3
]}

Almutairi, Layla A. ^{[4
]}

Begum, Ruhena ^{[1
]}

Sabrin, Mirza Synthia ^{[5
]}

Akanda, Md. Rashedunnabi ^{[6
]}

Hossain, Md. Mukter ^{[1
]}

Islam, Md. Rafiqul ^{[1
]}

Rahman, Md. Masudur ^{[7
]}

Rahman, Md. Mahfujur ^{[1
]}

机构：

[1] Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh

[2] Sylhet Agr Univ, Dept Anat & Histol, Sylhet 3100, Bangladesh

[3] King Khalid Univ, Coll Sci, Dept Biol, POB 960, Abha 61421, Saudi Arabia

[4] Princess Nourah bint Abdulrahman Univ, Coll Sci, Dept Biol, POB 84428, Riyadh 11671, Saudi Arabia

[5] Sher Ebangla Agr Univ, Fac Anim Sci & Vet Med, Dept Microbiol & Parasitol, Dhaka 1207, Bangladesh

[6] Sylhet Agr Univ, Dept Pharmacol & Toxicol, Sylhet 3100, Bangladesh

[7] Sylhet Agr Univ, Dept Pathol, Sylhet 3100, Bangladesh

来源：

PAKISTAN VETERINARY JOURNAL | 2024年 / 44卷 / 04期

关键词：

Captive wild birds; STEC; MDR; Migratory Birds; Virulent genes; E; coli; ANTIMICROBIAL RESISTANCE; ANTIBIOTIC-RESISTANCE; QUINOLONE RESISTANCE; SALMONELLA; INFECTIONS; CARRIERS; ANIMALS; STRAINS;

D O I：

10.29261/pakvetj/2024.264

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Multi-drug resistant-Shiga toxin producing Escherichia coli (MDR-STEC), poses considerable health risks for humans, animals and birds. Migratory and captive wild birds are known carriers of this pathogen. This study aimed to investigate prevalence of MDR-STEC along with its associated virulence genes from migratory and captive wild birds in Bangladesh. A total of 247 fecal specimens were obtained from both migratory (119) and captive wild birds (128) for the isolation and characterization of E. coli. Standard microbiological and biochemical methods were used for identification and Polymerase Chain Reaction (PCR) was employed for confirmation of E. coli isolates followed by disc diffusion method to determine antibiotic susceptibility. The overall E. coli prevalence was 80.97% (200/247; 95% CI: 75.5185.67), with a prevalence of 77.31% (92/119) in migratory birds and 84.38% (108/128) in captive wild birds. Among the 200 E. coli isolates, 53 (26.5%; 95% CI: 20.5-33.2) were identified as multidrug-resistant (MDR), with 21.7% (20/92) of MDR isolates originating from migratory birds and 30.6% (33/108) from captive wild birds. Only 24 (12%) isolates were positive for virulent gene stx2 whereas 167 (83.50%) isolates were positive for fimC. Among the beta-lactamase resistant genes, bla TEM (91.50%; 183/200) was found significantly (P<0.0001) higher than bla SHV (9.00%; 18/200). Among the antimicrobial resistant genes, 175 (87.50%) isolates were found positive for qnrS resistant gene. E. coli isolates of birds exhibited diverse phenotypic AMR patterns, with complete (100%) resistance to several antibiotics (ampicillin, ceftazidime, cefuroxime, and tetracycline) while being entirely sensitive to others (ceftriaxone, amikacin and aztreonam). This research underscores the concerning prevalence of E. coli strains having various virulent genes and resistant to multiple drugs among the wild birds. It emphasizes the immediate requirement for bridging wildlife and public health domains to address the threats posed by the antibiotic-resistant pathogens.

引用

页码：1120 / 1130

页数：11

共 62 条

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