Manufacturing exosomes for wound healing: Comparative analysis of culture media

被引:4
作者
Dao, Huy Hoang [1 ,5 ]
Nguyen, Thu-Huyen [1 ]
Hoang, Diem Huong [1 ]
Vu, Bach Duong [1 ]
Tran, Minh-Anh [1 ]
Le, Mai Thi [1 ,2 ]
Hoang, Nhung Thi My [2 ]
Bui, Anh Viet [1 ]
Than, Uyen Thi Trang [1 ,3 ]
Nguyen, Xuan-Hung [1 ,3 ,4 ]
机构
[1] Vinmec Healthcare Syst, Vinmec HiTech Ctr, Hanoi, Vietnam
[2] Vietnam Natl Univ, VNU Univ Sci, Fac Biol, Hanoi, Vietnam
[3] Vinmec Healthcare Syst, Vinmec VinUni Inst Immunol, Hanoi, Vietnam
[4] VinUniv, Coll Hlth Sci, Hanoi, Vietnam
[5] Univ Sci & Technol Hanoi, Vietnam Acad Sci & Technol, Hanoi, Vietnam
来源
PLOS ONE | 2024年 / 19卷 / 11期
关键词
MESENCHYMAL STEM-CELLS; GLYCOLYLNEURAMINIC ACID XENOANTIGEN; HEPATOCYTE GROWTH-FACTOR; HUMAN ADIPOSE-TISSUE; UMBILICAL-CORD; ANGIOGENESIS; SECRETOME; IL-7;
D O I
10.1371/journal.pone.0313697
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Mesenchymal stem cell (MSC)-derived exosomes (EXs) have emerged as promising therapeutic agents for wound healing. However, the optimal conditions for manufacturing MSC-derived EXs that maximize their wound-healing potential have yet to be established. Hence, we compared the efficacy of five different MSC culture media, including three different serum-free, a platelet-supplemented, and a fetal bovine serum-supplemented media, in exosome manufacturing for wound healing applications. Although umbilical cord-derived MSCs (UCMSCs) cultured in these media exhibited similar proliferation, morphology, MSC surface marker expression, and stemness, EXs derived from UCMSCs cultured in different culture media displayed varying levels of growth factors and cytokines. Notably, EXs derived from platelet-supplemented media (DM-PLT_EXs) exhibited significantly higher concentrations of keratinocyte growth factor (KGF), vascular endothelial growth factor (VEGF-A), platelet-derived growth factor (PDGF-BB), interleukin 6 (IL-6), interleukin 7 (IL-7), and interleukin 8 (IL-8) than EXs from other media. These differences correlated with the superior capability of DM-PLT_EXs to promote human skin fibroblast proliferation and stimulate angiogenesis of human umbilical vein endothelial cells, making them a more suitable choice for wound healing applications. Our findings emphasize the significance of the culture medium selection in tailoring the therapeutic potential of UCMSC-derived EXs for wound healing.
引用
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页数:18
相关论文
共 54 条
[1]   Exosomes in fetal bovine serum dampen primary macrophage IL-1β response to lipopolysaccharide (LPS) challenge [J].
Beninson, Lida A. ;
Fleshner, Monika .
IMMUNOLOGY LETTERS, 2015, 163 (02) :187-192
[2]   Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions [J].
Bhat, Samatha ;
Viswanathan, Pachaiyappan ;
Chandanala, Shashank ;
Prasanna, S. Jyothi ;
Seetharam, Raviraja N. .
SCIENTIFIC REPORTS, 2021, 11 (01)
[3]   Angiogenesis Analyzer for ImageJ - A comparative morphometric analysis of "Endothelial Tube Formation Assay" and "Fibrin Bead Assay" [J].
Carpentier, Gilles ;
Berndt, Sarah ;
Ferratge, Segolene ;
Rasband, Wayne ;
Cuendet, Muriel ;
Uzan, Georges ;
Albanese, Patricia .
SCIENTIFIC REPORTS, 2020, 10 (01)
[4]   Xeno-Free Strategies for Safe Human Mesenchymal Stem/Stromal Cell Expansion: Supplements and Coatings [J].
Cimino, M. ;
Goncalves, R. M. ;
Barrias, C. C. ;
Martins, M. C. L. .
STEM CELLS INTERNATIONAL, 2017, 2017
[5]   Interleukin-8: an autocrine/paracrine growth factor for human hematopoietic progenitors acting in synergy with colony stimulating factor-1 to promote monocyte-macrophage growth and differentiation [J].
Corre, I ;
Pineau, D ;
Hermouet, S .
EXPERIMENTAL HEMATOLOGY, 1999, 27 (01) :28-36
[6]   Comparison of multi-lineage cells from human adipose tissue and bone marrow [J].
De Ugarte, DA ;
Morizono, K ;
Elbarbary, A ;
Alfonso, Z ;
Zuk, PA ;
Zhu, M ;
Dragoo, JL ;
Ashjian, P ;
Thomas, B ;
Benhaim, P ;
Chen, I ;
Fraser, J ;
Hedrick, MH .
CELLS TISSUES ORGANS, 2003, 174 (03) :101-109
[7]   Differential Wound Healing Capacity of Mesenchymal Stem Cell-Derived Exosomes Originated From Bone Marrow, Adipose Tissue and Umbilical Cord Under Serum- and Xeno-Free Condition [J].
Diem Huong Hoang ;
Tu Dac Nguyen ;
Hoang-Phuong Nguyen ;
Xuan-Hung Nguyen ;
Phuong Thi Xuan Do ;
Van Duc Dang ;
Phuong Thi Minh Dam ;
Hue Thi Hong Bui ;
Mai Quynh Trinh ;
Duc Minh Vu ;
Nhung Thi My Hoang ;
Liem Nguyen Thanh ;
Uyen Thi Trang Than .
FRONTIERS IN MOLECULAR BIOSCIENCES, 2020, 7
[8]   Effects of Extracellular Vesicles Secreted by TGFP-Stimulated Umbilical Cord Mesenchymal Stem Cells on Skin Fibroblasts by Promoting Fibroblast Migration and ECM Protein Production [J].
Duc Minh Vu ;
Van-Tinh Nguyen ;
Thu Huyen Nguyen ;
Phuong Thi Xuan Do ;
Huy Hoang Dao ;
Do Xuan Hai ;
Nhi Thi Le ;
Xuan-Hung Nguyen ;
Uyen Thi Trang Than .
BIOMEDICINES, 2022, 10 (08)
[9]   The Role of Fibroblast Growth Factor (FGF) Signaling in Tissue Repair and Regeneration [J].
Farooq, Mariya ;
Khan, Abdul Waheed ;
Kim, Moon Suk ;
Choi, Sangdun .
CELLS, 2021, 10 (11)
[10]   A System of Cytokines Encapsulated in ExtraCellular Vesicles [J].
Fitzgerald, Wendy ;
Freeman, Michael L. ;
Lederman, Michael M. ;
Vasilieva, Elena ;
Romero, Roberto ;
Margolis, Leonid .
SCIENTIFIC REPORTS, 2018, 8