Developmental and Molecular Effects of C-Type Natriuretic Peptide Supplementation in In Vitro Culture of Bovine Embryos

被引:0
作者
Costa, Camila Bortoliero [1 ,2 ,3 ]
da Silva, Nathalia Covre [3 ]
Silva, Amanda Nespolo [4 ,5 ]
Pioltine, Elisa Mariano [2 ]
Dellaqua, Thaisy Tino [1 ]
Zangirolamo, Amanda Fonseca [3 ,6 ]
Meirelles, Flavio Vieira [5 ]
Seneda, Marcelo Marcondes [3 ,6 ]
Nogueira, Marcelo Fabio Gouveia [1 ,2 ]
机构
[1] Sao Paulo State Univ UNESP, Inst Biosci, Grad Program Pharmacol & Biotechnol, BR-18618970 Botucatu, SP, Brazil
[2] Sao Paulo State Univ UNESP, Sch Sci Humanities & Languages, Dept Biol Sci, BR-19806900 Assis, SP, Brazil
[3] Univ Londrina UEL, Dept Vet Med, Lab Anim Reprod, BR-86057970 Londrina, PR, Brazil
[4] Univ Sao Paulo, Grad Program Anat Domest & Wild Anim, BR-13635000 Pirassununga, SP, Brazil
[5] Univ Sao Paulo, Coll Anim Sci & Food Engn, Dept Vet Med, BR-13635000 Pirassununga, SP, Brazil
[6] Univ Londrina UEL, Natl Inst Sci & Technol Dairy Prod Chain INCT LEIT, BR-86057970 Londrina, PR, Brazil
基金
巴西圣保罗研究基金会;
关键词
C-type natriuretic peptide; embryonic metabolism; NPR2; transcript abundance; cattle; ENDOPLASMIC-RETICULUM; COMPETENCE; EXPRESSION; COMPLEXES; OOCYTES; GENE; IVM;
D O I
10.3390/ijms252010938
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The use of C-type natriuretic peptide (CNP) in the interaction with the oocyte and in the temporary postponement of spontaneous meiosis resumption has already been well described. However, its action in pre-implantation developmental-stage embryos is yet to be understood. Thus, our study aimed to detect the presence of the canonical CNP receptor (natriuretic peptide receptor, NPR2) in germinal vesicle (GV)-, metaphase II (MII)-, presumptive zygote (PZ)-, morula (MO)-, and blastocyst (BL)-stage embryos and, later, to observe possible modulations on the embryos when co-cultured with CNP. In Experiment I, we detected and quantified NPR2 on the abovementioned embryo stages. Further, in Experiment II, we intended to test different concentrations (100, 200, or 400 nM of CNP) at different times of inclusion in the in vitro culture (IVC; inclusion from the beginning, i.e., day 1, or from day 5). In Experiment III, 400 nM of CNP was used on day 1 (D1) in the IVC, which was not demonstrated to be embryotoxic, and it showed potentially promising results in the blastocyst production rate when compared to the control. Thus, we analyzed the embryonic development rates of bovine embryos (D7) and hatching kinetics (D7, D8, and D9). Subsequently, morula and blastocyst were collected and evaluated for transcript abundance of their competence and quality (apoptosis, oxidative stress, proliferation, and differentiation) and lipid metabolism. Differences with probabilities less than p < 0.05, and/or fold change (FC) > 1.5, were considered significant. We demonstrate the presence of NPR2 until the blastocyst development stage, when there was a significant decrease in membrane receptors. There was no statistical difference in the production rate after co-culture with 400 nM CNP. However, when we evaluated the abundance of morula transcripts, there was an upregulated transcription in ADCY6 (p = 0.057) and downregulated transcripts in BMP15 (p = 0.013), ACAT1 (p = 0.040), and CASP3 (p = 0.082). In addition, there was a total of 12 transcriptions in morula that presented variation FC > 1.5. In blastocysts, the treatment with CNP induced upregulation in BID, CASP3, SOX2, and HSPA5 transcripts and downregulation in BDNF, NLRP5, ELOVL1, ELOVL4, IGFBP4, and FDX1 transcripts (FC > 1.5). Thus, our study identified and quantified the presence of NPR2 in bovine pre-implantation embryos. Furthermore, 400 nM of CNP in IVC, a concentration not previously described in the literature, modulated some transcripts related to embryonic metabolism, and this was not embryotoxic morphologically.
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页数:15
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共 39 条
  • [1] The endoplasmic reticulum: a multifunctional signaling organelle
    Berridge, MJ
    [J]. CELL CALCIUM, 2002, 32 (5-6) : 235 - 249
  • [2] Supplementing in vitro embryo production media by NPPC and sildenafil affect the cytoplasmic lipid content and gene expression of bovine cumulus-oocyte complexes and embryos
    Botigelli, Ramon Cesar
    Razza, Eduardo Montanari
    Pioltine, Elisa Mariano
    Fontes, Patricia Kubo
    Lancellotti Schwarz, Katia Regina
    Verde Leal, Claudia Lima
    Gouveia Nogueira, Marcelo Fabio
    [J]. REPRODUCTIVE BIOLOGY, 2018, 18 (01) : 66 - 75
  • [3] Bone morphogenetic protein 15 and fibroblast growth factor 10 enhance cumulus expansion, glucose uptake, and expression of genes in the ovulatory cascade during in vitro maturation of bovine cumulus-oocyte complexes
    Caixeta, Ester S.
    Sutton-McDowall, Melanie L.
    Gilchrist, Robert B.
    Thompson, Jeremy G.
    Price, Christopher A.
    Machado, Mariana F.
    Lima, Paula F.
    Buratini, Jose
    [J]. REPRODUCTION, 2013, 146 (01) : 27 - 35
  • [4] Antioxidant Nobiletin Enhances Oocyte Maturation and Subsequent Embryo Development and Quality
    Cajas, Yulia N.
    Canon-Beltran, Karina
    de Guevara, Magdalena Ladron
    Millan de la Blanca, Maria G.
    Ramos-Ibeas, Priscila
    Gutierrez-Adan, Alfonso
    Rizos, Dimitrios
    Gonzalez, Encina M.
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (15) : 1 - 18
  • [5] Influence of cAMP modulator supplementation of in vitro culture medium on Bos taurus indicus embryos
    Costa, Camila Bortoliero
    Lunardelli, Paula Alvares
    Fontes, Patricia Kubo
    Sudano, Mateus Jose
    Gouveia Nogueira, Marcelo Fabio
    Alfieri, Amauri Alcindo
    Ferreira, Christina Ramires
    de Lima, Camila Bruna
    Rafagnin Marinho, Luciana Simoes
    Seneda, Marcelo Marcondes
    [J]. THERIOGENOLOGY, 2020, 141 : 134 - 141
  • [6] Modulating the lipid profile of blastocyst cell membrane with DPPC multilamellar vesicles
    De Rossi, Hugo
    Bortoliero Costa, Camila
    Rodrigues-Rossi, Luana Teixeira
    Barros Nunes, Giovana
    Spinosa Cheles, Doris
    Maran Pereira, Isabella
    Rocha, Daniele F. O.
    Feitosa, Eloi
    Colnaghi Simionato, Ana Valeria
    Zoccal Mingoti, Gisele
    Benites Aoki, Pedro Henrique
    Gouveia Nogueira, Marcelo Fabio
    [J]. ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, 2022, 50 (01) : 158 - 167
  • [7] L-arginine affects the IVM of cattle cumulus-oocyte complexes
    Dubeibe, D. F.
    Caldas-Bussiere, M. C.
    Maciel, V. L., Jr.
    Sampaio, W. V.
    Quirino, C. R.
    Goncalves, P. B. D.
    De Cesaro, M. P.
    Faes, M. R.
    de Carvalho, C. S. Paes
    [J]. THERIOGENOLOGY, 2017, 88 : 134 - 144
  • [8] Bona fide gene expression analysis of samples from the bovine reproductive system by microfluidic platform
    Fontes, Patricia Kubo
    Souza Castilho, Anthony Cesar
    Razza, Eduardo Montanari
    Gouveia Nogueira, Marcelo Fabio
    [J]. ANALYTICAL BIOCHEMISTRY, 2020, 596
  • [9] Natriuretic Peptide Precursor C Delays Meiotic Resumption and Sustains Gap Junction-Mediated Communication in Bovine Cumulus-Enclosed Oocytes
    Franciosi, Federica
    Coticchio, Giovanni
    Lodde, Valentina
    Tessaro, Irene
    Modina, Silvia C.
    Fadini, Rubens
    Dal Canto, Mariabeatrice
    Renzini, Mario Mignini
    Albertini, David F.
    Luciano, Alberto M.
    [J]. BIOLOGY OF REPRODUCTION, 2014, 91 (03)
  • [10] Grealy M, 1999, J REPROD FERTIL, V116, P355, DOI 10.1530/jrf.0.1160355