Identification and Genome Characterization of Begomovirus and Satellite Molecules Associated with Lettuce (Lactuca sativa L.) Leaf Curl Disease

被引：1
|
作者：
Tang, Yafei [1 ,2 ]
Du, Mengdan [1 ,2 ,3 ]
Li, Zhenggang [1 ,2 ]
Yu, Lin [1 ,2 ]
Lan, Guobing [1 ,2 ]
Ding, Shanwen [1 ,2 ]
Farooq, Tahir [1 ]
He, Zifu [1 ,2 ]
She, Xiaoman [1 ,2 ]
机构：
[1] Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China
[2] Minist Agr & Rural Affairs, South China Key Lab Green Prevent & Control Fruits, Guangzhou 510640, Peoples R China
[3] South China Agr Univ, Coll Plant Protect, Guangzhou 510640, Peoples R China
来源：
PLANTS-BASEL | 2025年 / 14卷 / 05期
基金：
中国国家自然科学基金;
关键词：
lettuce leaf curl disease; pepper leaf curl Yunnan virus; tomato leaf curl China betasatellite; ageratum yellow vein alphasatellite; new geminialphasatellite; TURNIP-MOSAIC-VIRUS; BIG-VEIN-VIRUS; INFECTING LETTUCE; DNA-BETA; TRANSCRIPTION; PRIMERS; NUCLEAR;
D O I：
10.3390/plants14050782
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Lettuce (Lactuca sativa L.) plants showing leaf curl and vein enation symptoms were found in Yunnan province, China. PCR detection with genus-specific primers revealed that symptomatic lettuce plants were infected with Begomovirus. The full-length viral component and satellite molecules were obtained by RCA, restriction enzyme digestion, PCR, cloning and DNA sequencing. A viral component (YN-2023-WJ) and three satellite molecules (YN-2023-WJ-alpha1, YN-2023-WJ-alpha2 and YN-2023-WJ-beta) were obtained from diseased lettuce plants. YN-2023-WJ exhibited the highest nt identity at 97.1% with pepper leaf curl Yunnan virus isolated from cigar plants. YN-2023-WJ-beta displayed the highest nt identity at 93.9% with tomato leaf curl China betasatellite. YN-2023-WJ-alpha1 showed the highest nt identity at 94.7% with ageratum yellow vein alphasatellite. YN-2023-WJ-alpha2 shared the highest nt identity at 75.6% with gossypium mustelinum symptomless alphasatellite and vernonia yellow vein Fujian alphasatellite. Based on the threshold for the classification of Begomovirus, Betasatellite and Alphasatellite, YN-2023-WJ was designated as a new isolate of PepLCYnV, YN-2023-WJ-beta as a new isolate of ToLCCNB and YN-2023-WJ-alpha1 as a new member of AYVA, whereas YN-2023-WJ-alpha2 was identified as a new geminialphasatellite species, for which the name pepper leaf curl Yunnan alphasatellite (PepLCYnA) is proposed. To the best of our knowledge, this is the first report of L. sativa L. infection by PepLCYnV associated with ToLCCNB, AYVA and PepLCYnA, and L. sativa L. is a new host plant of Begomovirus.
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页数：10
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