LncRNA MBNL1-AS1 functions as an alternative atherosclerosis biomarker in elderly hypertensive patients and regulates vascular smooth muscle cell function

被引：2
作者：
Song, Yanxu [1 ]
Zhu, Xingguang [2 ]
Cai, Xiangang [3 ]
Yu, Yinling [4 ]
Hu, Di [5 ]
机构：
[1] Qingdao Univ, Dept Med, Qingdao, Peoples R China
[2] Shandong Second Prov Gen Hosp, Dept Cardiovasc Surg, Jinan, Peoples R China
[3] Huazhong Univ Sci & Technol, Union Jiangbei Hosp, Dept Cardiol, Wuhan, Peoples R China
[4] Huazhong Univ Sci & Technol, Union Jiangbei Hosp, Dept Geriatr, Wuhan, Peoples R China
[5] Yijishan Hosp, Wannan Med Coll, Affiliated Hosp 1, Dept Cardiovasc Med, Zheshan West Rd 2, Wuhu 241001, Peoples R China
来源：
MINERVA CARDIOLOGY AND ANGIOLOGY | 2025年 / 73卷 / 03期
关键词：
Atherosclerosis; Hypertension; Muscle; smooth; vascular; PROGRESSION;
D O I：
10.23736/S2724-5683.24.06619-5
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
BACKGROUND: The clinical role of long non-coding RNA (MBNL1-AS1) in diagnosing atherosclerosis (AS) risks of hypertensive patients and the effects of MBNL1-AS1 on vascular smooth muscle cells (VSMCs) triggered by angiotensin II (Ang II) were investigated. METHODS: The hypertensive patients were recruited to assess MBNL1-AS1 expression. The ROC curve and Spearman analysis was performed for the significance of MBNL1-AS1. Human VSMCs were treated with Ang II(10-5 mol/L) to establish a hypertensive cell model. MTT and Transwell chamber were used in proliferative and migratory detection of cell models. Targets of MBNL1-AS1 were verified by luciferase activity. Functional enrichment of shared targets of miR-424-5p was researched by GO and KEGG analysis. RESULTS: An increase of MBNL1-AS1 was observed in patients with increased carotid intima-media thickness (cIMT). MBNL1-AS1 could predict the risk of AS and related to cIMT levels. The knockdown of MBNL1-AS1 mitigated the influence of Ang II on cellular proliferation and migration by inhibiting miR-424-5p. Enrichment analysis corroborated that targets of miR-424-5p were mainly involved in serine/threonine kinase activity, MAPK signaling pathway, and PI3K-Akt signaling pathway. CONCLUSIONS: MBNL1-AS1/miR-424-5p axis was connected with the progression of AS induced by hypertension.
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页码：258 / 266
页数：9
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