Integrated electrophysiological and genomic profiles of single cells reveal spiking tumor cells in human glioma

被引：6

作者：

Curry, Rachel N. ^{[1
,2
]}

Ma, Qianqian ^{[3
,4
]}

McDonald, Malcolm F. ^{[2
,5
,6
]}

Ko, Yeunjung ^{[2
,7
,8
]}

Srivastava, Snidgdha ^{[3
,5
,9
]}

Chin, Pey-Shyuan ^{[4
]}

He, Peihao ^{[2
,7
,10
]}

Lozzi, Brittney ^{[11
]}

Athukuri, Azwal ^{[8
]}

Jing, Junzhan ^{[3
]}

Wang, Su ^{[8
]}

Harmanci, Arif O. ^{[12
]}

Arenkiel, Benjamin ^{[4
,9
]}

Jiang, Xiaolong ^{[3
,4
,13
]}

Deneen, Benjamin ^{[1
,2
,6
,7
,8
,10
]}

Harmanci, Akdes Serin ^{[7
,8
]}

Rao, Ganesh ^{[7
,8
]}

机构：

[1] Baylor Coll Med, Integrat Mol & Biomed Sci Grad Program, Houston, TX 77030 USA

[2] Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA

[3] Texas Childrens Hosp, Jan & Dan Duncan Neurol Res Inst, Houston, TX 77030 USA

[4] Baylor Coll Med, Dept Neurosurg, Houston, TX 77030 USA

[5] Baylor Coll Med, Med Scientist Training Program, Houston, TX USA

[6] Baylor Coll Med, Program Dev Dis Models & Therapeut, Houston, TX 77030 USA

[7] Baylor Coll Med, Ctr Canc Neurosci, Houston, TX 77030 USA

[8] Baylor Coll Med, Dept Neurosurg, Houston, TX 77030 USA

[9] Baylor Coll Med, Dept Human & Mol Genet, Houston, TX USA

[10] Baylor Coll Med, Program Canc Cell Biol, Houston, TX 77030 USA

[11] Baylor Coll Med, Program Genet & Genom, Houston, TX USA

[12] Univ Texas Hlth Sci Ctr, McWilliams Sch Biomed Informat, Houston, TX USA

[13] Baylor Coll Med, Dept Ophthalmol, Houston, TX 77030 USA

来源：

CANCER CELL | 2024年 / 42卷 / 10期

关键词：

RNA-SEQ; OLIGODENDROCYTES; GLIA; POPULATION; MECHANISMS; PROMOTES; ORIGIN; ATLAS; IDH1;

D O I：

10.1016/j.ccell.2024.08.009

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Prior studies have described the complex interplay that exists between glioma cells and neurons; however, the electrophysiological properties endogenous to glioma cells remain obscure. To address this, we employed Patch-sequencing (Patch-seq) on human glioma specimens and found that one-third of patched cells in IDH mutant (IDHmut) mut ) tumors demonstrate properties of both neurons and glia. To define these hybrid cells (HCs), which fire single, short action potentials, and discern if they are of tumoral origin, we developed the single cell rule association mining (SCRAM) computational tool to annotate each cell individually. SCRAM revealed that HCs possess select features of GABAergic neurons and oligodendrocyte precursor cells, and include both tumor and non-tumor cells. These studies characterize the combined electrophysiological and molecular properties of human glioma cells and describe a cell type in human glioma with unique electrophysiological and transcriptomic properties that may also exist in the non-tumor brain.

引用

页码：1713 / 1728.e6

页数：23

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