Effect of Bacillus velezensis T23 solid-state fermentation product on growth, gut and liver health, and gut microbiota of common carp ( Cyprinus carpio) )

被引：0

作者：

Chen, Xingyu ^{[1
]}

Liu, Shubin ^{[2
]}

Teame, Tsegay ^{[1
,3
]}

Luo, Jia ^{[1
,4
]}

Liu, Yao ^{[1
,4
]}

Zhou, Qingwen ^{[1
,4
]}

Ding, Qianwen ^{[2
]}

Yao, Yuanyuan ^{[2
]}

Yang, Yalin ^{[2
]}

Ran, Chao ^{[2
]}

Zhang, Zhen ^{[2
,5
]}

Zhou, Zhigang ^{[1
,6
]}

机构：

[1] Chinese Acad Agr Sci, Inst Feed Res, China Norway Joint Lab Fish Gut Microbiota, Beijing 100081, Peoples R China

[2] Chinese Acad Agr Sci, Feed Res Inst, Minist Agr & Rural Affairs, Inst Feed Res,Key Lab Feed Biotechnol, Beijing 100081, Peoples R China

[3] Tigray Agr Res Inst, Mekelle, Tigray, Ethiopia

[4] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China

[5] Univ British Columbia, Fac Land & Food Syst, Vancouver, BC, Canada

[6] Kunpeng Inst Modern Agr Foshan, Foshan 528226, Peoples R China

来源：

AQUACULTURE | 2025年 / 596卷

基金：

中国国家自然科学基金;

关键词：

Bacillus velezensis; Intestinal microbiota; Solid state fermentation product; Gut and liver health; BLUNT SNOUT BREAM; HIGH-FAT; INTESTINAL MICROBIOTA; LARGEMOUTH BASS; PROBIOTICS; IMMUNITY; DIET; ANTIBIOTICS; EXPRESSION; MECHANISM;

D O I：

10.1016/j.aquaculture.2024.741733

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Nowadays, the fermented products of probiotics have been playing a significant role in aquaculture and become an emerging research interest. This study evaluated the 0, 0.2, 0.3 and 0.4 g/kg solid state fermentation product of Bacillus velezensis T23 supplemented diets (39 % crude protein and 10 % crude fat) on the growth, lipid metabolism, liver and intestinal health, and gut microbiota of Cyprinus carpio (2.51 +/- 0.01 g) by physiobiochemical, histomorphological and gut microbiome methods. The results showed that in the 0.4 T23 group, the weight gain was significantly increased (p p < 0.05), compared to the control. . In the 0.3 and 0.4 T23 groups, the expressions of lipogenesis-related genes (fas, fas , acc, srebp and ppar gamma) were significantly down-regulated, while the expressions of lipolysis-related ( ppar alpha, lpl, , fabp1 and apo-po) ) genes were up-regulated. Furthermore, the levels of serum ALT and AST were decreased notably (p p < 0.05), whereas T-AOC and SOD activity were increased significantly in the 0.3 and 0.4 T23 groups, compared with the control. Pro-inflammatory genes ( nf-kb , tnf-alpha, and il-1 beta) ) were downregulated in 0.3 and 0.4 T23 groups (p p < 0.05). Anti-inflammatory related genes ( il-10 and tgf-beta) ) were upregulated in 0.3 T23 groups. This result had been supported by liver histomorphology that T23 diets had decreased the levels of liver health biomarkers and intestinal injury and improve the hepatic antioxidant capacity. Dietary supplementation of T23 at a level of 0.3 g/kg increased alpha-diversity- diversity and the relative abundance of Fusobacteriota (phylum) and Cetobacterium (genus). The ratio of "(Fusobacteriota+Firmicutes+Bacteroidota)/ +Firmicutes +Bacteroidota)/ Proteobacteria" in 0.3 and 0.4 T23 groups was significantly increased, compared with the control group. Hence, this study clarified that the addition of solid state fermentation product of B. velezensis T23 to the diet (0.3 and 0.4 g/kg) of common carp improves inflammatory response, liver and gut health, and modulates the intestinal microbiota of carp positively.

引用

页数：11

共 68 条

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