Key Neuropeptides Regulating Molting in Pacific White Shrimp (Penaeus vannamei): Insights from Transcriptomic Analysis

被引：0
作者：
Li, Xianliang [1 ]
Li, Yunjiao [2 ]
Li, Zecheng [1 ]
Chen, Hu [1 ]
机构：
[1] Hainan Univ, Sanya Inst Breeding & Multiplicat, Hainan Aquaculture Breeding Engn Res Ctr, Sch Marine Biol & Fisheries,Key Lab Trop Hydrobiol, Haikou 570228, Peoples R China
[2] Sichuan Acad Agr Sci, Fisheries Res Inst, Yibin 644000, Peoples R China
来源：
ANIMALS | 2025年 / 15卷 / 04期
关键词：
<italic>Penaeus vannamei</italic>; molting; neuropeptides; transcriptomic neuropeptides; transcriptomic; CLEAVAGE SITES; CHITIN; EXPRESSION; HORMONES; PROTEINS; BURSICON; CHH;
D O I：
10.3390/ani15040540
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Molting is a vital physiological process essential for the growth and development of Penaeus vannamei, with significant implications for aquaculture productivity. This study aimed to identify neuropeptide-related genes involved in molting through transcriptomic analysis. RNA sequencing of pre-molt and post-molt samples revealed 1203 differentially expressed genes (DEGs). Functional enrichment analysis indicated that these genes play significant roles in cuticle formation and molting regulation. Among the DEGs, 243 were predicted to be neuropeptides based on the presence of signal peptides and the absence of transmembrane domains. Five key neuropeptide genes-PvCHH, PvMIH, PvEH I, PvCDA I, and PvCDA II-were identified as critical regulators of molting. Their role was further validated through RT-qPCR analysis, confirming their close association with the molting process. These genes were highlighted in this study as pivotal factors driving molting in P. vannamei. The neuropeptides identified in this research are anticipated to offer valuable insights into the regulation of molting. Additionally, their synthetic products hold promise for improving molting consistency in shrimp aquaculture.
引用
收藏
页数：15
相关论文
共 52 条
  • [1] Wang H., Teng M., Liu P., Zhao M., Wang S., Hu J., Bao Z., Zeng Q., Selection signatures of Pacific White Shrimp Litopenaeus vannamei revealed by whole-genome resequencing analysis, Front. Mar. Sci, 9, (2022)
  • [2] The State of World Fisheries and Aquaculture 2024–Blue Transformation in Action, (2024)
  • [3] Villarreal H., Shrimp farming advances, challenges, and opportunities, J. World Aquacult. Soc, 54, pp. 1092-1095, (2023)
  • [4] Alfaro-Montoya J., Braga A., Umana-Castro R., Research frontiers in penaeid shrimp reproduction: Future trends to improve commercial production, Aquaculture, 503, pp. 70-87, (2019)
  • [5] Emerenciano M.G., Rombenso A.N., Vieira F., Martins M.A., Coman G.J., Truong H.H., Noble T.H., Simon C.J., Intensification of penaeid shrimp culture: An applied review of advances in production systems, nutrition and breeding, Animals, 12, (2022)
  • [6] Lemos D., Weissman D., Moulting in the grow-out of farmed shrimp: A review, Rev. Aquacult, 13, pp. 5-17, (2021)
  • [7] Gao Y., Zhang X., Wei J., Sun X., Yuan J., Li F., Xiang J., Whole transcriptome analysis provides insights into molecular mechanisms for molting in Litopenaeus vannamei, PLoS ONE, 10, (2015)
  • [8] Diwan A., Current progress in shrimp endocrinology—A review, Indian. J. Exp. Biol, 43, pp. 209-223, (2005)
  • [9] Wei J., Zhang X., Yu Y., Huang H., Li F., Xiang J., Comparative transcriptomic characterization of the early development in Pacific white shrimp Litopenaeus vannamei, PLoS ONE, 9, (2014)
  • [10] Corteel M., Dantas-Lima J., Wille M., Alday-Sanz V., Pensaert M., Sorgeloos P., Nauwynck H., Moult cycle of laboratory-raised Penaeus (Litopenaeus) vannamei and P. monodon, Aquacult. Int, 20, pp. 13-18, (2012)
123456