A Ratiometric Fluorescence Nano pH Biosensor for Live-Cell Imaging Using Cerasome

被引：0

作者：

Zhang, Zhongqiao ^{[1
]}

Luo, Xiaoshan ^{[2
]}

Wang, Xuanbo ^{[2
]}

Liu, Meng ^{[3
]}

Yue, Xiuli ^{[4
]}

Zheng, Zhaozhu ^{[2
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

[2] Soochow Univ, Natl Engn Lab Modern Silk, Suzhou 215123, Peoples R China

[3] Soochow Univ, Suzhou Med Coll, Cyrus Tang Hematol Ctr, Collaborat Innovat Ctr Hematol, Suzhou 215123, Peoples R China

[4] Harbin Inst Technol, Sch Municipal & Environm Engn, Harbin 150001, Peoples R China

来源：

BIOSENSORS-BASEL | 2025年 / 15卷 / 02期

关键词：

ratiometric fluorescence; pH sensor; nanoparticles; cerasome; live cells; SOL-GEL; SENSORS; MATRIX; DYE;

D O I：

10.3390/bios15020114

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of a robust and biocompatible pH-sensing platform is critical for monitoring intracellular processes and diagnosing diseases. Here, we present a smart ultrastable ratiometric fluorescence nano pH sensor based on silica-coated liposome nanoparticles (cerasome, 138.4 nm). The sensor integrates pH-sensitive dye, pyranine, within cerasome, achieving enhanced photostability, sensitivity, and biocompatibility. Its unique ratiometric design enables precise pH monitoring with minimal photobleaching and quenching, covering a linear detection range of pH 6.25-8.5. The hybrid nanoparticles exhibit high morphological stability, making them suitable for real-time intracellular pH measurement. This novel platform shows great promise for applications in cellular biology, disease diagnosis, and therapeutic monitoring, offering a versatile tool for biomedical research.

引用

页数：12

共 43 条

[11] Zhou J., Ren Y., Nie Y., Jin C., Park J., Zhang J.X.J., Dual fluorescent hollow silica nanofibers for in situ pH monitoring using an optical fiber, Nanoscale Adv, 5, pp. 2180-2189, (2023)
[12] Chen Y., Zhu C., Cen J., Bai Y., He W., Guo Z., Ratiometric detection of pH fluctuation in mitochondria with a new fluorescein/cyanine hybrid sensor, Chem. Sci, 6, pp. 3187-3194, (2015)
[13] Huang Z., Li Q., Xue H., Liao W., Feng Y., Yuan J., Tao L., Wei Y., Synthesis of an aggregation-induced emission (AIE) dye with pH-sensitivity based on tetraphenylethylene-pyridine for fluorescent nanoparticles and its applications in bioimaging and in vitro anti-tumor effect, Colloids Surf. B Biointerfaces, 234, (2024)
[14] Qi J., Liu D., Liu X., Guan S., Shi F., Chang H., He H., Yang G., Fluorescent pH Sensors for Broad-Range pH Measurement Based on a Single Fluorophore, Anal. Chem, 87, pp. 5897-5904, (2015)
[15] Gui R.J., Jin H., Dual-emitting fluorescence ratiometric nanoprobes of in-vitro/in-vivo pH from constructions to sensing, imaging and therapeutic applications, J. Photochem. Photobiol. C: Photochem. Rev, 58, (2024)
[16] Islam S., Atomic site structural functionality and thermal stability of phenol red encapsulated zincite incorporated silica-anatase microspheres for opto-chemical pH sensing, Mater. Chem. Phys, 290, (2022)
[17] Cao J.T., Xie M.T., Gao X.Y., Zhang Z.Y., Wang J.G., Zhou W.J., Guan W.J., Lu C., Charge neutralization strategy to construct salt-tolerant and cell-permeable nanoprobes: Application in ratiometric sensing and imaging of intracellular pH, Anal. Chem, 93, pp. 15159-15166, (2021)
[18] Kromer C., Katz A., Feldmann I., Laux P., Luch A., Tschiche H.R., A targeted fluorescent nanosensor for ratiometric pH sensing at the cell surface, Sci. Rep, 14, (2024)
[19] Li L., Zhdanov A.V., Papkovsky D.B., Advanced multimodal solid-state optochemical pH and dual pH/O<sub>2</sub> sensors for cell analysis, Sens. Actuators B Chem, 371, (2022)
[20] Islam S., Bakhtiar H., Naseem S., Abd Aziz M.S.B., Bidin N., Riaz S., Ali J., Surface functionality and optical properties impact of phenol red dye on mesoporous silica matrix for fiber optic pH sensing, Sens. Actuators A Phys, 276, pp. 267-277, (2018)

← 1 2 3 4 5 →