A Ratiometric Fluorescence Nano pH Biosensor for Live-Cell Imaging Using Cerasome

被引：0

作者：

Zhang, Zhongqiao ^{[1
]}

Luo, Xiaoshan ^{[2
]}

Wang, Xuanbo ^{[2
]}

Liu, Meng ^{[3
]}

Yue, Xiuli ^{[4
]}

Zheng, Zhaozhu ^{[2
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

[2] Soochow Univ, Natl Engn Lab Modern Silk, Suzhou 215123, Peoples R China

[3] Soochow Univ, Suzhou Med Coll, Cyrus Tang Hematol Ctr, Collaborat Innovat Ctr Hematol, Suzhou 215123, Peoples R China

[4] Harbin Inst Technol, Sch Municipal & Environm Engn, Harbin 150001, Peoples R China

来源：

BIOSENSORS-BASEL | 2025年 / 15卷 / 02期

关键词：

ratiometric fluorescence; pH sensor; nanoparticles; cerasome; live cells; SOL-GEL; SENSORS; MATRIX; DYE;

D O I：

10.3390/bios15020114

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of a robust and biocompatible pH-sensing platform is critical for monitoring intracellular processes and diagnosing diseases. Here, we present a smart ultrastable ratiometric fluorescence nano pH sensor based on silica-coated liposome nanoparticles (cerasome, 138.4 nm). The sensor integrates pH-sensitive dye, pyranine, within cerasome, achieving enhanced photostability, sensitivity, and biocompatibility. Its unique ratiometric design enables precise pH monitoring with minimal photobleaching and quenching, covering a linear detection range of pH 6.25-8.5. The hybrid nanoparticles exhibit high morphological stability, making them suitable for real-time intracellular pH measurement. This novel platform shows great promise for applications in cellular biology, disease diagnosis, and therapeutic monitoring, offering a versatile tool for biomedical research.

引用

页数：12

共 43 条

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