A brief guide for gene delivery to the brain using adeno-associated viral vectors

被引：1
作者：
Han, Seungwan [1 ,2 ]
Yang, Eun Mo [1 ,2 ]
Hur, Eun-Mi [1 ,2 ,3 ,4 ,5 ]
机构：
[1] Seoul Natl Univ, Coll Vet Med, Lab Neurosci, Seoul 08826, South Korea
[2] Seoul Natl Univ, Coll Vet Med, FOUR Future Vet Med Leading Educ & Res Ctr BK21,La, Seoul, South Korea
[3] Seoul Natl Univ, Res Inst Vet Sci, Coll Vet Med, Seoul 08826, South Korea
[4] Seoul Natl Univ, Comparat Med Dis Res Ctr, Sci Res Ctr, Seoul 08826, South Korea
[5] Seoul Natl Univ, Coll Nat Sci, Interdisciplinary Program Neurosci, Seoul 08826, South Korea
来源：
MOLECULES AND CELLS | 2025年 / 48卷 / 03期
基金：
新加坡国家研究基金会;
关键词：
Gene delivery; Intracranial injection; Recombinant adeno-associated viral vector; Stereotaxic surgery; VIRUS VECTOR; TRANSGENE EXPRESSION; RAT-BRAIN; TRANSDUCTION; THERAPY; SEROTYPES; MOUSE; EVOLUTION; PROMOTER; MODEL;
D O I：
10.1016/j.mocell.2025.100189
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The advent of recombinant adeno-associated viral (rAAV) vector-mediated gene delivery has accelerated the comprehensive analysis and manipulation of the nervous system owing to its ability to regulate gene expression in a spatiotemporal manner, thereby facilitating the study of brain physiology and the investigation of the pathophysiology of neurological disorders. Here, we provide a concise guide to stereotaxic gene delivery into the mouse brain using rAAV vectors. Key considerations for designing a customized rAAV vector are discussed, along with an overview of the surgical procedures of intracranial stereotaxic injection. This article aims to assist neuroscientists in establishing experimental setups for genetic manipulation in the mouse brain. (c) 2025 The Author(s). Published by Elsevier Inc. on behalf of Korean Society for Molecular and Cellular Biology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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页数：8
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