Enhanced osteogenic differentiation of human periodontal ligament cells by mature osteoclasts

被引：0
作者：
Suamphan, Sumit [1 ]
Makeudom, Anupong [2 ]
Krisanaprakornkit, Suttichai [2 ]
Meekhantong, Pimphorn [2 ]
Dechtham, Ekapong [2 ]
Leethanakul, Chidchanok [1 ,3 ]
机构：
[1] Prince Songkla Univ, Fac Dent, Dept Prevent Dent, Orthodont Sect, Hat Yai 90112, Songkhla, Thailand
[2] Mae Fah Luang Univ, Sch Dent, Chiang Rai 57100, Thailand
[3] Prince Songkla Univ, Fac Dent, Ctr Excellence Oral Hlth, Hat Yai 90112, Songkhla, Thailand
来源：
JOURNAL OF ORAL BIOSCIENCES | 2025年 / 67卷 / 02期
关键词：
Osteoclast; Osteogenesis; Osteoprotegerin; Periodontal ligament; RANKL; ANTIMICROBIAL PEPTIDE; STEM-CELLS; FIBROBLASTS; PROTEIN;
D O I：
10.1016/j.job.2025.100632
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Objective: Several in vitro studies have shown that reverse signaling from osteoclasts regulates osteoblast differentiation and mineralization. However, none of these studies have reported the effects of this signaling pathway on periodontal ligament (PDL) cells. Therefore, in this study, we aimed to investigate the interaction between receptor activators of nuclear factor kappa B (RANK) released from mature human osteoclasts and the membranous RANK ligand (RANKL) in human PDL cells. Methods: Multinucleated mature human osteoclasts were differentiated from peripheral blood mononuclear cells upon incubation with recombinant macrophage colony-stimulating factor and RANKL. Mature osteoclasts and human PDL cells were characterized. A mature osteoclast-conditioned medium (OC-CM) was used to induce osteogenic differentiation of PDL cells. Mechanistic analysis of RANK-RANKL reverse signaling were conducted to determine the regulation of osteogenic induction using conditioned medium from mature osteoclasts treated with GW4869 (GW-OC-CM) or PDL cells pretreated with recombinant human osteoprotegerin (OPG). Results: OC-CM significantly upregulated the mRNA expression of osteogenic genes and enhanced the osteogenic differentiation and biomineralization of PDL cells (p < 0.05). GW-OC-CM significantly reduced the expression of osteogenic genes, osteogenic differentiation, and biomineralization in PDL cells (p < 0.05). Similarly, the pretreatment of PDL cells with OPG before OC-CM treatment significantly reduced the osteogenic induction of PDL cells (p < 0.05). Conclusion: Mature osteoclasts can induce osteogenesis in human PDL cells via RANK-RANKL reverse signaling.
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页数：10
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共 31 条
  • [1] Yasuda H., Discovery of the RANKL/RANK/OPG system, J Bone Miner Metab, 39, pp. 2-11, (2021)
  • [2] Seo M., Miura M., Gronthos S., Bartold M., Batouli S., Brahim J., Et al., Investigation of multipotent postnatal stem cells from human periodontal ligament, Lancet, 364, pp. 149-155, (2004)
  • [3] Liu J., Zhao Z., Ruan J., Weir D., Ma T., Ren K., Et al., Stem cells in the periodontal ligament differentiated into osteogenic, fibrogenic and cementogenic lineages for the regeneration of the periodontal complex, J Dent, 92, (2020)
  • [4] Arima M., Hasegawa D., Yoshida S., Mitarai H., Tomokiyo A., Hamano S., Et al., R-spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/β-catenin signaling pathway, J Periodontal Res, 54, pp. 143-153, (2019)
  • [5] Manokawinchoke J., Pavasant P., Sawangmake C., Limjeerajarus N., Limjeerajarus N., Egusa H., Et al., Intermittent compressive force promotes osteogenic differentiation in human periodontal ligament cells by regulating the transforming growth factor-β pathway, Cell Death Dis, 10, (2019)
  • [6] Li Y., Zhan Q., Bao M., Yi J., L Y., Biomechanical and biological responses of periodontium in orthodontic tooth movement: up-date in a new decade, Int J Oral Sci, 13, (2021)
  • [7] Bloemen V., Schoenmaker T., de Vries J., Everts V., Direct cell-cell contact between periodontal ligament fibroblasts and osteoclast precursors synergistically increases the expression of genes related to osteoclastogenesis, J Cell Physiol, 222, pp. 565-573, (2010)
  • [8] Ikebuchi Y., Aoki S., Honma M., Hayashi M., Sugamori Y., Khan M., Kariya Y., Kato G., Tabata Y., Penninger J.M., Udagawa N., Aoki K., Suzuki H., Coupling of bone resorption and formation by RANKL reverse signalling, Nature, 561, 7722, pp. 195-200, (2018)
  • [9] Liao M., Guan Z., Yang J., Ma Y., Dai J., Liang C., Et al., Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells, BMC Oral Health, 22, (2022)
  • [10] Stessuk T., Husch J., Hermens T., Hofmann S., van den Beucken P., Osteogenic differentiation driven by osteoclasts and macrophages, J Immunol Regen Med, 12, (2021)
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