Impact of iso-osmotic acclimation in condition on ex vitro performance of white turmeric (Curcuma zedoaria [Cristm.] Roscoe)

被引：0

作者：

Tisarum, Rujira ^{[1
]}

Bayaraa, Unenzaya ^{[2
]}

Sotesaritkul, Thanyaporn ^{[1
]}

Samphumphuang, Thapanee ^{[1
]}

Singh, Harminder Pal ^{[3
]}

Cha-um, Suriyan ^{[1
]}

机构：

[1] Natl Ctr Genet Engn & Biotechnol BIOTEC, Natl Sci & Technol Dev Agcy NSTDA, 113 Paholyothin Rd, Bangkok 12120, Pathum Thani, Thailand

[2] Mongolian Acad Sci, Inst Biol, Plant Biotechnol Lab, Peace Ave 54B, Ulaanbaatar 13330, Mongolia

[3] Panjab Univ, Fac Sci, Dept Environm Studies, Chandigarh 160014, India

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2025年

关键词：

Curcuminoids; <italic>Curcuma zedoaria</italic>; Iso-osmotic acclimation; Physiological adaptation; Osmopriming; Sugar alcohol; IN-VITRO; OSMOTIC-STRESS; PLANTLETS; SUCROSE; MANNITOL; GROWTH; CULTURE; ACCUMULATION; L; TRANSITION;

D O I：

10.1007/s11627-024-10510-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Low survival rate, poor adaptation to ex vitro environments, and time required for hardening plants to adapt to fluctuating environments of field trials are major barriers to micropropagation techniques. Three iso-osmotic treatments (6% sucrose, 4% mannitol, and 3% sucrose + 2.5% mannitol) along with 3% sucrose as control were established to study the in vitro hardening process of Curcuma zedoaria [Cristm.] Roscoe (white turmeric) plants. They were transferred to ex vitro environments and subsequently cultivated in greenhouse conditions. The growth and physiology of C. zedoaria plants primed with 3% sucrose + 2.5% mannitol treatment were significantly adapted during in vitro and ex vitro conditions. Pseudostem height and root length in acclimatized plantlets under 3% sucrose + 2.5% mannitol treatments were compacted as compared to control, resulting in a small plant in the ex vitro adaptation for 6 wk. Morphological and physiological traits of aboveground and underground parameters of acclimatized-plantlets derived from 3% sucrose + 2.5% mannitol treatments were maximized, leading to high yield of curcuminoids (229.4 mg plant-1) in dry rhizomes when cultivated under greenhouse microenvironments for 9 mo. The osmopriming of in-vitro C. zedoaria plantlets using culture medium containing 3% sucrose + 2.5% mannitol was indicated as a suitable protocol for ex vitro adaptation and resulted in high curcuminoid content in the rhizomes at the harvesting stage. In summary, the plantlets of C. zedoaria micropropagated using culture medium containing 3% sucrose + 2.5% mannitol can readily acclimatize under ex vitro conditions and subsequently develop as healthy plants in greenhouse conditions. Based on the results, in vitro acclimation using iso-osmotic treatment is recommended for white turmeric and furthermore this technique may be implemented in other rhizomatous plant species, especially within the Zingeberaceae family.

引用

页数：12