Dual-Responsive Nanoparticles for Smart Drug Delivery: A NIR Light-Sensitive and Redox-Reactive PEG-PCL-Based System

被引：8
作者：
Ferrentino, Nancy [1 ]
Kohlan, Taha Behroozi [2 ]
Mehrtashfar, Shokoufeh [2 ,3 ]
Finne-Wistrand, Anna [2 ]
Pappalardo, Daniela [1 ]
机构：
[1] Univ Sannio, Dipartimento Sci & Tecnol, I-82100 Benevento, Italy
[2] KTH Royal Inst Technol, Dept Fiber & Polymer Technol, Sch Engn Sci Chem Biotechnol & Hlth, SE-10044 Stockholm, Sweden
[3] Univ Tehran, Coll Sci, Dept Biotechnol, Tehran 141556455, Iran
来源：
BIOMACROMOLECULES | 2024年 / 25卷 / 12期
基金：
瑞典研究理事会;
关键词：
TRIBLOCK COPOLYMER NANOPARTICLES; POLYMERIC NANOPARTICLES; NILE RED; IN-VITRO; AGGREGATION; DOXORUBICIN; RELEASE;
D O I：
10.1021/acs.biomac.4c00889
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Stimuli-responsive polymeric nanoparticles (NPs) can serve as smart drug delivery systems (DDSs) by triggering drug release upon external or internal stimuli. A dual-responsive DDS made of a triblock poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) (PCL-SS-PEG-SS-PCL) copolymer, bearing disulfide bonds between PCL and PEG, was synthesized. The copolymer was functionalized with coumarin and sensitive to near-infrared (NIR) light irradiation, while the S-S bonds could be cleaved by GSH (10 mM). Characterization was achieved by nuclear magnetic resonance, size exclusion chromatography, and Fourier transform infrared analyses. Nile Red (NR)-loaded NPs were prepared through self-assembly of the copolymer in water and analyzed by dynamic light scattering and field-emission scanning electron microscopy. The NR release upon ultraviolet (UV)/NIR light irradiation as well as by GSH concentrations was monitored by using fluorescence spectroscopy, while simultaneous exposure to UV/NIR light and intracellular GSH concentration led to faster NR release. AlamarBlue assay showed satisfactory cell viability of the NR-loaded NPs, while their cellular uptake in human dermal fibroblast cells was investigated by fluorescence microscopy and fluorescence emission measurements.
引用
收藏
页码：7660 / 7673
页数：14
相关论文
共 46 条
[1]   A New Two-Photon-Sensitive Block Copolymer Nanocarrier [J].
Babin, Jerome ;
Pelletier, Maxime ;
Lepage, Martin ;
Allard, Jean-Francois ;
Morris, Denis ;
Zhao, Yue .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (18) :3329-3332
[2]   The role of glutathione in cancer [J].
Balendiran, GK ;
Dabur, R ;
Fraser, D .
CELL BIOCHEMISTRY AND FUNCTION, 2004, 22 (06) :343-352
[3]   Polymeric Nanoparticles for Drug Delivery [J].
Beach, Maximilian A. ;
Nayanathara, Umeka ;
Gao, Yanting ;
Zhang, Changhe ;
Xiong, Yijun ;
Wang, Yufu ;
Such, Georgina K. .
CHEMICAL REVIEWS, 2024, 124 (09) :5505-5616
[4]   Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models [J].
Bonnier, F. ;
Keating, M. E. ;
Wrobel, T. P. ;
Majzner, K. ;
Baranska, M. ;
Garcia-Munoz, A. ;
Blanco, A. ;
Byrne, H. J. .
TOXICOLOGY IN VITRO, 2015, 29 (01) :124-131
[5]   Effect of ionic strength on aggregation of nile red and coumarin 30 in aqueous medium: primary kinetic salt effect or salting-out effect? [J].
Chattopadhyay, Nitin ;
Das, Arindam .
RSC ADVANCES, 2023, 13 (36) :25159-25168
[6]   Recent Advances in Stimuli-Responsive Release Function Drug Delivery Systems for Tumor Treatment [J].
Ding, Chendi ;
Tong, Ling ;
Feng, Jing ;
Fu, Jiajun .
MOLECULES, 2016, 21 (12)
[7]   Amphiphilic Biodegradable PEG-PCL-PEI Triblock Copolymers for FRET-Capable in Vitro and in Vivo Delivery of siRNA and Quantum Dots [J].
Endres, Thomas ;
Zheng, Mengyao ;
Kilic, Ayse ;
Turowska, Agnieszka ;
Beck-Broichsitter, Moritz ;
Renz, Harald ;
Merkel, Olivia M. ;
Kissel, Thomas .
MOLECULAR PHARMACEUTICS, 2014, 11 (04) :1273-1281
[8]   Preparation and in vivo pharmacokinetics of curcumin-loaded PCL-PEG-PCL triblock copolymeric nanoparticles [J].
Feng, Runliang ;
Song, Zhimei ;
Zhai, Guangxi .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2012, 7 :4089-4098
[9]   Poly(ε-caprolactone)-poly(ethylene glycol) Tri-Block Copolymer as Quercetin Delivery System for Human Colorectal Carcinoma Cells: Synthesis, Characterization and In Vitro Study [J].
Ferrentino, Nancy ;
Romano, Maria Preziosa ;
Zappavigna, Silvia ;
Abate, Marianna ;
Del Vecchio, Vitale ;
Romano, Dario ;
Germinario, Chiara ;
Grifa, Celestino ;
Filosa, Rosanna ;
Pappalardo, Daniela .
POLYMERS, 2023, 15 (05)
[10]   Redox-Responsive Disulfide Cross-Linked PLA-PEG Nanoparticles [J].
Fuoco, Tiziana ;
Pappalardo, Daniela ;
Finne-Wistrand, Anna .
MACROMOLECULES, 2017, 50 (18) :7052-7061
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