Bottom-emitting striped microLED array light source for uniform optical sectioning structured illumination microscopy

被引:0
作者
Durnan, Oliver [1 ]
Saladrigas, Catherine A. [2 ]
Kumar, Vikrant [1 ]
Ogunmwonyi, Ikponmwosa Stephen [1 ]
Bright, Victor M. [3 ]
Gopinath, Juliet T. [2 ,4 ]
Kymissis, Ioannis [1 ]
机构
[1] Columbia Univ, Dept Elect Engn, New York, NY 10027 USA
[2] Univ Colorado Boulder, Dept Elect Comp & Energy Engn, Boulder, CO USA
[3] Univ Colorado Boulder, Dept Mech Engn, Boulder, CO USA
[4] Univ Colorado Boulder, Dept Phys, Boulder, CO USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
MicroLED light source; MicroLED microscopy; miniature microscopes; neural circuits; neural tissue imaging; optical sectioning; OS-; SIM; structured illumination microscopyfluorescence microscopy; structured illumination microscopy; DIODES;
D O I
10.1002/jsid.2069
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
We demonstrate a microLED light source for optical sectioning structured illumination microscopy (OS-SIM). A bottom-emitting design is employed to improve the uniformity and intensity of emission by removing the anode contact from the optical path. This design facilitates the addition of a potential equalizing and light-reflecting layer to the top surface of the light source. The irradiance, uniformity of light emission, and contrast ratio of the light source are characterized. A system demonstration is performed with the light source integrated into a fluorescence microscope and used for OS-SIM imaging.
引用
收藏
页码:482 / 490
页数:9
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