Identification of RppSLN from an Elite Landrace: A Major Locus Conferring Resistance to Southern Corn Rust in Maize (Zea mays L.)

被引:2
作者
Wang, Yufei [1 ,2 ]
Ma, Shuai [2 ,3 ]
Zhang, Dengfeng [2 ]
Li, Chunhui [2 ]
Chen, Lin [2 ]
Tang, Bin [2 ]
An, Yixin [2 ]
Liu, Xuyang [2 ]
He, Guanhua [2 ]
Shi, Yunsu [2 ]
Li, Yu [2 ]
Wang, Tianyu [2 ]
Yang, Deguang [1 ]
Li, Yongxiang [2 ]
机构
[1] Northeast Agr Univ, Coll Agr, Harbin 150006, Peoples R China
[2] Chinese Acad Agr Sci, Inst Crop Sci, State Key Lab Crop Gene Resources & Breeding, Beijing 100081, Peoples R China
[3] Beijing Acad Agr & Forestry Sci, Inst Grassland Flowers & Ecol, Beijing 100097, Peoples R China
来源
PLANTS-BASEL | 2024年 / 13卷 / 22期
关键词
maize (Zea mays L.); southern corn rust; landrace; RppSLN; GENE; TOOL; SEQ;
D O I
10.3390/plants13223227
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Southern corn rust (SCR) is one of the most destructive foliar diseases in maize (Zea mays L.), resulting in significant yield losses. Therefore, the continuous identification of disease-resistant germplasm and the deployment of resistant hybrids is essential for durably controlling SCR. The objective of this research was to identify and characterize resistance loci against SCR in maize to expand disease management strategies. Here, we identified a maize landrace with high resistance to SCR 'Silunuo' (SLN) approaching complete immunity. We backcrossed it with a susceptible inbred line, N531, to generate a stable SCR-resistant introgression line N531_R. By crossing it with F35 (a susceptible inbred line), we created a large F2 segregating population and mapped a major SCR-resistant locus on chromosome 10, known as RppSLN. Based on the genome assembly and annotation, we found that RppSLN harbors two NBS-LRR (nucleotide binding site-leucine-rich repeat) genes, namely Zmays10G000430 and Zmays10G000440. These NBS-LRR genes were significantly induced during artificial inoculation with Puccinia polysora, suggesting that they might be candidate genes collectively contributing to the resistance level at this locus. In conclusion, this study identified a major SCR resistance locus directly isolated from a landrace, providing valuable support and information for expanding new disease-resistant germplasms and promoting the utilization of landraces.
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页数:14
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