Color Change and Cytotoxic Effects of a Bleaching Gel With 22% Carbamide Peroxide Catalyzed by Manganese Oxide

被引：0

作者：

Ribeiro, Rafael Antonio de Oliveira ^{[1
]}

Peruchi, Victoria ^{[1
]}

Sonego, Leonardo Novaes Gaeta ^{[2
]}

Soares, Igor Paulino Mendes ^{[1
]}

Soares, Diana Gabriela ^{[3
]}

Hebling, Josimeri ^{[4
]}

Costa, Carlos Alberto de Souza ^{[2
]}

机构：

[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, Brazil

[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Araraquara, Brazil

[3] Sao Paulo Univ USP, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, Brazil

[4] Sao Paulo State Univ Unesp, Sch Dent, Dept Morphol Genet Orthodont & Pediat Dent, Araraquara, Brazil

来源：

JOURNAL OF ESTHETIC AND RESTORATIVE DENTISTRY | 2025年

基金：

瑞典研究理事会;

关键词：

cell culture; dental materials; tooth bleaching; toxicity; ODONTOBLAST; 10-PERCENT; EFFICACY; SENSITIVITY;

D O I：

10.1111/jerd.13401

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objectives: To evaluate the color change and trans-amelodentinal cytotoxicity of a 22% carbamide peroxide (CP) bleaching gel containing different concentration of manganese oxide (MnO2). Material and Methods: Enamel/dentin discs adapted to artificial pulp chambers were distributed according to treatments: CN-No treatment; CP22%-22%CP; CP22 + 2MnO(2)-22%CP + 2 mg/mLMnO(2); CP22% + 6MnO(2)-22%CP + 6 mg/mLMnO(2); CP22% + 10MnO(2)-22%CP + 10 mg/mLMnO(2) applied for 2 h for 15 days. Color change-CC (Delta E-00 and Delta WID) (n = 8) was determined at 5, 10, and 15-day periods (ANOVA/Sidak). Trans-amelodentinal cytotoxicity (TC) was assessed after applying the extracts to cultured MDPC-23 cells, which were analyzed concerning their viability (Vi) (n = 8) and oxidative stress (OxS) (n = 8). The amount of hydroxyl radical (OH center dot) (n = 4) generated by H2O2 degradation (ANOVA/Sidak), and trans-amelodentinal diffusion of H2O2 (n = 8) (ANOVA/Tukey) were also evaluated. Results: CP22 + 10MnO(2) presented higher CC than CP22 for both evaluated equations in all analysis periods (p <= 0.04). Among bleached groups, CP22 + 10MnO(2) presented the highest OH center dot generation (p <= 0.02) and the lowest trans-amelodentinal diffusion of H2O2 (p <= 0.0004). Therefore, MDPC-23 cells in CP22 + 10MnO(2) exhibited the lowest OxS (p < 0.0001) and consequently the highest Vi (p <= 0.0146). Conclusion: Besides increasing the bleaching efficacy, the strategy of adding 10 mg/mL of MnO2 to a 22%CP bleaching gel also reduces significantly the trans-amelodentinal diffusion of H2O2, diminishing the cytotoxicity caused by at-home bleaching therapy.

引用

页数：9