Color Change and Cytotoxic Effects of a Bleaching Gel With 22% Carbamide Peroxide Catalyzed by Manganese Oxide

被引:0
|
作者
Ribeiro, Rafael Antonio de Oliveira [1 ]
Peruchi, Victoria [1 ]
Sonego, Leonardo Novaes Gaeta [2 ]
Soares, Igor Paulino Mendes [1 ]
Soares, Diana Gabriela [3 ]
Hebling, Josimeri [4 ]
Costa, Carlos Alberto de Souza [2 ]
机构
[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Araraquara, Brazil
[3] Sao Paulo Univ USP, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, Brazil
[4] Sao Paulo State Univ Unesp, Sch Dent, Dept Morphol Genet Orthodont & Pediat Dent, Araraquara, Brazil
基金
瑞典研究理事会;
关键词
cell culture; dental materials; tooth bleaching; toxicity; ODONTOBLAST; 10-PERCENT; EFFICACY; SENSITIVITY;
D O I
10.1111/jerd.13401
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objectives: To evaluate the color change and trans-amelodentinal cytotoxicity of a 22% carbamide peroxide (CP) bleaching gel containing different concentration of manganese oxide (MnO2). Material and Methods: Enamel/dentin discs adapted to artificial pulp chambers were distributed according to treatments: CN-No treatment; CP22%-22%CP; CP22 + 2MnO(2)-22%CP + 2 mg/mLMnO(2); CP22% + 6MnO(2)-22%CP + 6 mg/mLMnO(2); CP22% + 10MnO(2)-22%CP + 10 mg/mLMnO(2) applied for 2 h for 15 days. Color change-CC (Delta E-00 and Delta WID) (n = 8) was determined at 5, 10, and 15-day periods (ANOVA/Sidak). Trans-amelodentinal cytotoxicity (TC) was assessed after applying the extracts to cultured MDPC-23 cells, which were analyzed concerning their viability (Vi) (n = 8) and oxidative stress (OxS) (n = 8). The amount of hydroxyl radical (OH center dot) (n = 4) generated by H2O2 degradation (ANOVA/Sidak), and trans-amelodentinal diffusion of H2O2 (n = 8) (ANOVA/Tukey) were also evaluated. Results: CP22 + 10MnO(2) presented higher CC than CP22 for both evaluated equations in all analysis periods (p <= 0.04). Among bleached groups, CP22 + 10MnO(2) presented the highest OH center dot generation (p <= 0.02) and the lowest trans-amelodentinal diffusion of H2O2 (p <= 0.0004). Therefore, MDPC-23 cells in CP22 + 10MnO(2) exhibited the lowest OxS (p < 0.0001) and consequently the highest Vi (p <= 0.0146). Conclusion: Besides increasing the bleaching efficacy, the strategy of adding 10 mg/mL of MnO2 to a 22%CP bleaching gel also reduces significantly the trans-amelodentinal diffusion of H2O2, diminishing the cytotoxicity caused by at-home bleaching therapy.
引用
收藏
页数:9
相关论文
共 50 条
  • [1] A novel carbamide peroxide polymeric nanoparticle bleaching gel: Color change and hydrogen peroxide penetration inside the pulp cavity
    Favoreto, Michael W.
    Madureira, Mariniella P.
    Hass, Viviane
    Maran, Bianca M.
    Parreiras, Sibelli O.
    Borges, Christiane P. F.
    Reis, Alessandra
    Loguercio, Alessandro D.
    JOURNAL OF ESTHETIC AND RESTORATIVE DENTISTRY, 2021, 33 (02) : 277 - 283
  • [2] Assessing tooth color change after repeated bleaching in vitro with a 10 percent carbamide peroxide gel
    Lenhard, M
    JOURNAL OF THE AMERICAN DENTAL ASSOCIATION, 1996, 127 (11): : 1618 - 1624
  • [3] EVALUATING TOOTH COLOR-CHANGE FROM CARBAMIDE PEROXIDE GEL
    GEGAUFF, AG
    ROSENSTIEL, SF
    LANGHOUT, KJ
    JOHNSTON, WM
    JOURNAL OF THE AMERICAN DENTAL ASSOCIATION, 1993, 124 (06): : 65 - 72
  • [4] The efficacy and safety of a 10% carbamide peroxide bleaching gel
    Matis, BA
    Cochran, MA
    Eckert, G
    Carlson, TJ
    QUINTESSENCE INTERNATIONAL, 1998, 29 (09): : 555 - 563
  • [5] Cytotoxic Effects of Different Concentrations of a Carbamide Peroxide Bleaching Gel on Odontoblast-Like Cells MDPC-23
    de Lima, Adriano Fonseca
    Rosetti Lessa, Fernanda Campos
    Gasparoto Mancini, Maria Nadir
    Hebling, Josimeri
    de Souza Costa, Carlos Alberto
    Marchi, Giselle Maria
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2009, 90B (02) : 907 - 912
  • [6] INFLUENCE OF SOLUTIONS WITH PIGMENTATION POTENTIAL ON TOOTH COLOR AFTER BLEACHING USING 22% CARBAMIDE PEROXIDE
    de Oliveira Correia, Ayla Macyelle
    Duarte Melo, Bruno Everthon
    Barros Cedraz, Juliana da Silva
    da Rocha, Daniel Maranha
    dos Santos, Natanael Barbosa
    de Mendonca Fragoso, Larissa Silveira
    BIOSCIENCE JOURNAL, 2017, 33 (04): : 1106 - 1112
  • [7] Manganese oxide increases bleaching efficacy and reduces the cytotoxicity of a 10% hydrogen peroxide bleaching gel
    de Oliveira Ribeiro, Rafael Antonio
    Zuta, Uxua Ortecho
    Mendes Soares, Igor Paulino
    Anselmi, Caroline
    Soares, Diana Gabriela
    Fraga Briso, Andre Luiz
    Hebling, Josimeri
    de Souza Costa, Carlos Alberto
    CLINICAL ORAL INVESTIGATIONS, 2022, 26 (12) : 7277 - 7286
  • [8] Manganese oxide increases bleaching efficacy and reduces the cytotoxicity of a 10% hydrogen peroxide bleaching gel
    Rafael Antonio de Oliveira Ribeiro
    Uxua Ortecho Zuta
    Igor Paulino Mendes Soares
    Caroline Anselmi
    Diana Gabriela Soares
    André Luiz Fraga Briso
    Josimeri Hebling
    Carlos Alberto de Souza Costa
    Clinical Oral Investigations, 2022, 26 : 7277 - 7286
  • [9] Effect of chemical activation of 10% carbamide peroxide gel in tooth bleaching
    Batista, Graziela Ribeiro
    Arantes, Paula Tamiao
    Attin, Thomas
    Wiegand, Annette
    Gomes Torres, Carlos Rocha
    INTERNATIONAL JOURNAL OF ESTHETIC DENTISTRY, 2013, 8 (01): : 104 - 117
  • [10] Color change after tooth bleaching with ozone and 10% ozonized carbamide peroxide for in-office use
    Ferreira Neves Dias, Thalyta dos Reis Furlani Zouain
    de Campos, Francisco Ubiratan Ferreira
    Turssi, Cecilia Pedroso
    do Amaral, Flavia Lucisano Botelho
    Franca, Fabiana Mantovani Gomes
    Basting, Roberta Tarkany
    MEDICAL GAS RESEARCH, 2022, 12 (03): : 100 - 106