Hidden species' diversity and phylogenetic relationships within the terrestrial isopod genus Tiroloscia (Crustacea: Isopoda: Oniscidea)

被引:0
作者
Gardini, Pietro [1 ,2 ]
Sabatelli, Simone [1 ,2 ]
Taiti, Stefano [3 ,4 ]
Audisio, Paolo [1 ,2 ]
机构
[1] Sapienza Univ Rome, Dept Biol & Biotechnol Charles Darwin, Viale Univ 32, I-00186 Rome, Italy
[2] Natl Biodivers Future Ctr NBFC, Palermo, Italy
[3] Natl Res Council IRET CNR, Res Inst Terr Ecosyst, Via Madonna del Piano 10, I-50019 Florence, Italy
[4] Univ Firenze, Museo Storia Nat, Sez Zool La Specola, Via Romana 17, I-50125 Florence, Italy
关键词
molecular phylogeny; species diversity; species delimitation; molecular clock; taxonomy; Isopoda; MOLECULAR PHYLOGENY; GREEK POPULATIONS; DNA-SEQUENCES; RIBOSOMAL DNA; IQ-TREE; EVOLUTION; DELIMITATION; TAXONOMY; REVEALS; PHILOSCIIDAE;
D O I
10.1093/zoolinnean/zlae173
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Defining species boundaries may result challenging and has been a widely debated issue in the last decades. In cases of cryptic or "pseudocryptic" species, molecular approaches can be a valuable tool to provide taxonomic hypotheses and significantly complement morphological taxonomy. Here, two mitochondrial and one nuclear markers were used to study the phylogenetic relationships within the genus Tiroloscia, paying particular attention to Tiroloscia exigua. Moreover, we used a molecular clock to define a time window for the diversification of the main lineages within the species and explore aspects of its evolutionary history. Finally, four species delimitation methods were applied to clarify taxonomy and validate species boundaries. We found strong evidence against the monophyly of Tiroloscia and a surprisingly high level of genetic diversity within Tiroloscia exigua, supported by morphology. Notably, five evolutionary lineages were identified within T. exigua, suggesting the presence of distinct taxonomic entities. Divergence time estimation places the onset of T. exigua diversification around the middle Miocene (similar to 12.2 Mya). Based on phylogenetic and morphological results, we propose the resurrection of Tiroloscia squamuligera as a valid species. Our results underscore the importance of molecular approaches to uncover hidden diversity, particularly in terrestrial isopods which may hide still underestimated biodiversity.
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页数:17
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