Molecular Insights into Cell-Mediated Immunity in Atypical Non-Ulcerated Cutaneous Leishmaniasis

被引：0

作者：

Batista, Luis Fabio S. ^{[1
]}

Pacheco, Carmen M. Sandoval ^{[1
]}

Flores, Gabriela V. Araujo ^{[1
]}

Ferreira, Frederico M. ^{[1
]}

Goncalves, Andre N. A. ^{[2
]}

Sosa-Ochoa, Wilfredo H. ^{[3
]}

da Matta, Vania L. R. ^{[1
]}

Gomes, Claudia M. C. ^{[1
]}

Zuniga, Concepcion ^{[4
]}

Corbett, Carlos E. P. ^{[1
]}

Jeffares, Daniel C. ^{[5
]}

Nakaya, Helder I. ^{[2
,6
]}

Silveira, Fernando T. ^{[7
]}

Laurenti, Marcia D. ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Pathol, Med Sch, BR-01246903 Sao Paulo, Brazil

[2] Univ Sao Paulo, Dept Clin & Toxicol Anal, BR-05508000 Sao Paulo, Brazil

[3] Natl Autonomous Univ Honduras, Microbiol Res Inst, Tegucigalpa 11101, Honduras

[4] Natl Autonomous Univ Honduras, Dept Hlth Surveillance, Tegucigalpa 05005, Honduras

[5] Univ York, York Biomed Res Inst, Dept Biol, York YO31 5DD, England

[6] Hosp Israelita Albert Einstein, Sao Paulo, Brazil

[7] Evandro Chagas Inst, BR-66093020 Belem, Brazil

来源：

MICROORGANISMS | 2025年 / 13卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

atypical non-ulcerated cutaneous leishmaniasis; visceral leishmaniasis; <italic>L.</italic> (<italic>L.</italic>) <italic>infantum chagasi</italic>; transcriptome; cell-mediated immune response; HUMAN DENDRITIC CELLS; T-CELLS; EXPRESSION; CHAGASI; PATHWAY; INHIBITION; ACTIVATION; RESPONSES; INFANTUM; DISTINCT;

D O I：

10.3390/microorganisms13020413

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Leishmania (Leishmania) infantum chagasi infections range from asymptomatic (AS) to severe visceral leishmaniasis (VL). One of the manifestations is an atypical non-ulcerated cutaneous leishmaniasis (NUCL), which occurs in some locations of Central America with few cases of VL. We conducted a transcriptomic analysis of cell-mediated immunity (CMI) on blood samples from NUCL, AS, VL patients from Amapala, Honduras, and healthy controls. RNA-seq revealed a similar perturbation of gene expression in NUCL and AS. Eight gene signatures of CMI were found in NUCL involved in CD8+ T lymphocyte infiltration, reactive oxygen species generation, PD-1 receptor ligand, inflammasome assembly, chemotaxis, complement receptor and suppressor immune cell infiltration. NUCL was distinguished from VL by its up-regulation of differently expressed genes (DEGs) related to T lymphocyte exhaustion, adhesion and transmigration of leukocytes, and down-regulation of oxidative stress genes. In contrast, VL exhibited up-regulated DEGs involved in antigen cross-presentation, and similar to VL from Brazil, down-regulated DEGs involved in innate immunity. Corroborating the transcriptome findings, both the Leishmanin skin test, and the immunopathology of NUCL skin lesion defined NUCL as a proinflammatory condition, intermediate between the AS and VL clinical outcomes. That condition may be the underlying element for the benign nature of the NUCL.

引用

页数：20

共 70 条

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