Polystyrene-Poly(acrylic acid) Block Copolymers for Encapsulation of Butyrylcholinesterase into Injectable Nanoreactors

被引:0
|
作者
Fetin, Petr A. [1 ]
Zorin, Ivan M. [1 ]
Shaihutdinova, Zukhra M. [2 ,3 ]
Masson, Patrick [2 ]
Pashirova, Tatiana N. [2 ,3 ]
机构
[1] St Petersburg State Univ, Inst Chem, Dept Macromol Chem, 7-9 Universitetskaya Nab, St Petersburg 199034, Russia
[2] Kazan Fed Univ, Inst Fundamental Med & Biol, 18 Kremlyovskaya St, Kazan 420008, Russia
[3] RAS, Arbuzov Inst Organ & Phys Chem, FRC Kazan Sci Ctr, Arbuzov Str 8, Kazan 420088, Russia
基金
俄罗斯科学基金会;
关键词
butyrylcholinesterase; bioscavenger; polymersomes; self-assembly; enzyme activity; MULTIPLE MORPHOLOGIES; VESICLES; RELEASE; ACETYLCHOLINESTERASE; POLYMERSOMES; INHIBITION; DELIVERY;
D O I
10.3390/biom14121555
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The article is devoted to the creation of enzymatic nanoreactors based on polystyrene-block-poly(acrylic acid) (PS-b-PAA) copolymers containing bioscavengers capable of neutralizing toxic esters both in the body and in the environment. Block copolymers of different amphiphilicity, hydrophilicity and molecular weights were synthesized and characterized using gel permeation chromatography, NMR and UV spectroscopy. Polymeric nanocontainers in the absence and presence of human butyrylcholinesterase were made by film hydration and characterized by dynamic light scattering and microscopy methods. Enzyme activity was determined using the Ellman method. For the first time, factors that need to be taken into account for the creation of effective enzymatic nanoreactors based on PS-b-PAA are presented. The data obtained open up the possibility of PS-b-PAA nanoreactor use for future in vivo bioscavenger studies.
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页数:18
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