Inhibitory mechanism of chlorogenic acid on α-glucosidase and evaluation of its glucose consumption in HepG2 cells

被引：0
|
作者：
Jiang, Hua [1 ]
Wang, Yueyue [1 ]
Li, Jun [1 ]
Guo, Hezhe [1 ]
Wang, Lu [1 ]
Li, Jiaxing [1 ]
Wang, Xiuzhen [1 ]
Zhang, Yiqing [1 ]
机构：
[1] Henan Univ Sci & Technol, Sch Chem & Chem Engn, Luoyang 471000, Peoples R China
来源：
JOURNAL OF MOLECULAR STRUCTURE | 2025年 / 1331卷
关键词：
alpha-glucosidase inhibitory activity; Chlorogenic acid; Molecular docking; Glucose consumption; HepG2; cells; KINETICS; DERIVATIVES;
D O I：
10.1016/j.molstruc.2025.141607
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
There is an increasing demand for plant-derived antidiabetic drugs. This study systematically evaluated the potential of chlorogenic acid as an alpha-glucosidase inhibitor and its influence on glucose metabolism in liver HepG2 cells. Kinetic analysis revealed that chlorogenic acid suppressed alpha-glucosidase activity through the mixed-type mode and monophasic process. Fluorescence quenching and circular dichroism data demonstrated that the alpha-glucosidase-chlorogenic acid complex was formed. As revealed by molecular docking, chlorogenic acid showed main interactions with amino acid residues near the alpha-glucosidase active site, mainly including hydrogen bonding and hydrophobic interactions. Chlorogenic acid also demonstrated favorable glucose consumption in HepG2 cells with minimal cytotoxicity. Our findings validate its potential as an antidiabetic nutraceutical.
引用
收藏
页数：11
相关论文
共 50 条
  • [21] Antigenotoxic effects of quercetin, rutin and ursolic acid on HepG2 cells: Evaluation by the comet assay
    Ramos, A. A.
    Lima, C. F.
    Pereira, M. L.
    Fernandes-Ferreira, M.
    Pereira-Wilson, C.
    TOXICOLOGY LETTERS, 2008, 177 (01) : 66 - 73
  • [22] Evaluation of the Inhibitory Effects of Genipin on the Fluoxetine-Induced Invasive and Metastatic Model in Human HepG2 Cells
    Tian, Yu-Syuan
    Chen, Kuan-Chou
    Zulkefli, Nor Diana
    Maner, Rida S.
    Hsieh, Chiu-Lan
    MOLECULES, 2018, 23 (12):
  • [23] Inhibitory effects of Oren-gedoku-to and its components on cholesteryl ester synthesis in cultured human hepatocyte HepG2 cells: Evidence from the cultured HepG2 cells and in vitro assay of ACAT
    Yotsumoto, H
    Yanagita, T
    Yamamoto, K
    Ogawa, Y
    Cha, JY
    Mori, Y
    PLANTA MEDICA, 1997, 63 (02) : 141 - 145
  • [24] Synthesis of tetracyclic oxindoles and evaluation of their ?-glucosidase inhibitory and glucose consumption -promoting activity
    Hao, Lei
    Ma, Yujiao
    Zhao, Lianbo
    Zhang, Yinan
    Zhang, Xinying
    Ma, Ying
    Dodd, Robert H.
    Sun, Hua
    Yu, Peng
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2020, 30 (14)
  • [25] Effects of Phycocyanin on Glucose Metabolism in Insulin-Resistant HepG2 Cells
    Li F.
    Zhang W.
    Hao S.
    Yang Q.
    Li Q.
    Liu Y.
    Journal of Food Science and Technology (China), 2023, 41 (03): : 54 - 63+84
  • [26] Cantharidin content determination and its inhibitory effect on human hepatoma HepG2 cells.
    Lin, Yisheng
    Lia, Yanhao
    Peng, Zhenpeng
    Yu, Shilong
    Lin, Shuangfeng
    Huc, Xiaoshu
    BIOMEDICAL RESEARCH-INDIA, 2016, 27 (02): : 533 - 536
  • [27] Limonoids isolated from fruits of Swietenia macrophylla king enhance glucose consumption in insulin-resistant HepG2 cells via activating PPARγ
    Duan, Jing-Yu
    Wang, Yong-Jian
    Chen, Wei
    Zhao, Yang-Qi
    Bai, Zhong-Hui
    He, Liang-Liang
    Zhang, Chun-Ping
    JOURNAL OF FOOD BIOCHEMISTRY, 2021, 45 (04)
  • [28] Modulation of Lipogenesis and Glucose Consumption in HepG2 Cells and C2C12 Myotubes by Sophoricoside
    Wu, Chongming
    Luan, Hong
    Wang, Shuai
    Zhang, Xue
    Wang, Ran
    Jin, Lifeng
    Guo, Peng
    Chen, Xi
    MOLECULES, 2013, 18 (12) : 15624 - 15635
  • [29] Inhibitory Effect of Styrylpyrone Extract of Phellinus linteus on Hepatic Steatosis in HepG2 Cells
    Chiu, Chun-Hung
    Chen, Ming-Yao
    Lieu, Jun-Jie
    Chen, Chin-Chu
    Chang, Chun-Chao
    Chyau, Charng-Cherng
    Peng, Robert Y.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (04)
  • [30] MiR-34a overexpression enhances the inhibitory effect of doxorubicin on HepG2 cells
    Zheng, Shun-Zhen
    Sun, Ping
    Wang, Man-Ping
    Liu, Yong
    Gong, Wei
    Liu, Jun
    WORLD JOURNAL OF GASTROENTEROLOGY, 2019, 25 (22) : 2752 - 2762
12345