Immunogenicity and Protective Efficacy of an mRNA Vaccine Targeting HSV-2 UL41 in Mice

被引:0
|
作者
Mou, Tangwei [1 ]
Zhao, Yu [1 ]
Jia, Jie [1 ]
Gao, Kai-Cheng [1 ]
Li, Shao-You [1 ]
Kuang, Yi-Qun [1 ]
机构
[1] Kunming Med Univ, Affiliated Hosp 1, Res Ctr Clin Med, Kunming 650032, Peoples R China
基金
中国国家自然科学基金;
关键词
mRNA vaccines; HSV-2; UL41; protein; immune response; protective effects; HERPES-SIMPLEX-VIRUS; T-CELL RESPONSES; GENITAL HERPES; INFECTION; CD4; TEGUMENT;
D O I
10.3390/vaccines13030271
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background: Herpes simplex virus 2 (HSV-2) is the primary cause of sexually transmitted genital ulcerative diseases, for which no effective prophylactic vaccine is currently available. However, the identification of appropriate targets for an HSV-2 mRNA vaccine remains an area requiring further investigation. Methods: The immunogenicity and protective effects of an HSV-2 UL41 mRNA vaccine were evaluated in a BALB/c mouse model. The mice were intramuscularly immunized twice, followed by HSV-2 infection at 28 days post boost. Clinical signs were monitored daily, and the viral load and tissue inflammation were assessed on days 1, 4, and 7 post infection. Dendritic cell (DC) activation in spleen tissue was analyzed via transcriptome sequencing. Results: A comparison of the clinical, immunological, and pathological characteristics of the groups that were immunized with the UL41 mRNA vaccine and then infected with HSV2, along with the control groups, revealed that the vaccine elicited both cellular and humoral immunity, inhibited viral replication, suppressed the inflammatory response, and provided protective effects against the virus in vivo. Furthermore, in vitro assays of DC expansion revealed that the vaccine immunization increased the induction of DCs from splenic cells. Transcriptomic analysis of these DCs revealed the activation of immune signaling pathways. Conclusions: Our study suggests that the UL41 mRNA vaccine may provide effective protection against HSV-2-related diseases and holds promise as a potential mRNA vaccine candidate.
引用
收藏
页数:18
相关论文
共 50 条
  • [41] JuvImmune is a novel vaccine adjuvant that enhances protection of mice from lethal HSV-2 infection following immunization
    Strasser, Jane
    Cardin, Rhonda
    Chalk, Claudia
    Ireland, James
    Fairman, Jeff
    Bernstein, David
    ANTIVIRAL RESEARCH, 2007, 74 (03) : A42 - A42
  • [42] Immunogenicity and Protective Efficacy in Mice and Hamsters of a β-Propiolactone Inactivated Whole Virus SARS-CoV Vaccine
    Roberts, Anjeanette
    Lamirande, Elaine W.
    Vogel, Leatrice
    Baras, Benoit
    Goossens, Genevieve
    Knott, Isabelle
    Chen, Jun
    Ward, Jerrold M.
    Vassilev, Ventzislav
    Subbarao, Kanta
    VIRAL IMMUNOLOGY, 2010, 23 (05) : 509 - 519
  • [43] Immunogenicity and protective efficacy of a Plasmodium yoelii Hsp60 DNA vaccine in BALB/c mice
    Sanchez, GI
    Sedegah, M
    Rogers, WO
    Jones, TR
    Sacci, J
    Witney, A
    Carucci, DJ
    Kumar, N
    Hoffman, SL
    INFECTION AND IMMUNITY, 2001, 69 (06) : 3897 - 3905
  • [44] Safety immunogenicity and protective efficacy in mice of a new cell-cultured Lister smallpox vaccine candidate
    Ferrier-Rembert, Audrey
    Drillien, Robert
    Meignier, Bernard
    Garin, Daniel
    Crance, Jean-Marc
    VACCINE, 2007, 25 (49) : 8290 - 8297
  • [45] Immunogenicity and protective efficacy of a rhesus adenoviral vaccine targeting conserved COVID-19 replication transcription complex
    Dagotto, Gabriel
    Ventura, John D.
    Martinez, David R.
    Anioke, Tochi
    Chung, Benjamin S.
    Siamatu, Mazuba
    Barrett, Julia
    Miller, Jessica
    Schafer, Alexandra
    Yu, Jingyou
    Tostanoski, Lisa H.
    Wagh, Kshitij
    Baric, Ralph S.
    Korber, Bette
    Barouch, Dan H.
    NPJ VACCINES, 2022, 7 (01)
  • [46] Immunogenicity and protective efficacy of a rhesus adenoviral vaccine targeting conserved COVID-19 replication transcription complex
    Gabriel Dagotto
    John D. Ventura
    David R. Martinez
    Tochi Anioke
    Benjamin S. Chung
    Mazuba Siamatu
    Julia Barrett
    Jessica Miller
    Alexandra Schäfer
    Jingyou Yu
    Lisa H. Tostanoski
    Kshitij Wagh
    Ralph S. Baric
    Bette Korber
    Dan H. Barouch
    npj Vaccines, 7
  • [47] Immunogenicity and Protective Efficacy of Baculovirus-Expressed SARS-CoV-2 Envelope Protein in Mice as a Universal Vaccine Candidate
    Oguzoglu, Tuba cigdem
    Hanifehnezhad, Alireza
    Khabbazi, Saber Delpasand
    Karayel-Hacioglu, Ilke
    Kaynarcalidan, Onur
    Firat, Zehra
    Filazi, Nazlican
    Erdem-Sahinkesen, Eda
    Gul, Buket
    Karabulut, Muhammed Cesim
    Koba, Enes
    Adiguzel, Ece
    Senlik, Elif Irem
    Korkulu, Emrah
    Demirden, Cansu
    Sahinkesen, Ilker
    Ceylan, Ahmet
    Muratoglu, Hacer
    Vural, Sevil
    Demirbag, Zihni
    Ozkul, Aykut
    VACCINES, 2024, 12 (09)
  • [48] Immunogenicity and protective efficacy of a DNA vaccine inducing optimal expression of the SARS-CoV-2 S gene in hACE2 mice
    Li, Zhuo-Xin
    Feng, Sheng
    Zhang, He
    Zhuang, Xin-Yu
    Shang, Chao
    Sun, Shi-Yu
    Han, Ji-Cheng
    Xie, Yu-Biao
    Zhang, Jin-Yong
    Wang, Wei
    Li, Cheng-Hui
    Zhao, Guan-Yu
    Hao, Peng-Fei
    Ma, Jun-Xian
    Gao, Yan
    Zeng, Jia-Qing
    Tian, Ming-Yao
    Ha, Zhuo
    Lu, Hui-Jun
    Jin, Ning-Yi
    ARCHIVES OF VIROLOGY, 2022, 167 (12) : 2519 - 2528
  • [49] Immunogenicity and protective efficacy of a DNA vaccine inducing optimal expression of the SARS-CoV-2 S gene in hACE2 mice
    Zhuo-xin Li
    Sheng Feng
    He Zhang
    Xin-yu Zhuang
    Chao Shang
    Shi-yu Sun
    Ji-cheng Han
    Yu-biao Xie
    Jin-yong Zhang
    Wei Wang
    Cheng-hui Li
    Guan-yu Zhao
    Peng-fei Hao
    Jun-xian Ma
    Yan Gao
    Jia-qing Zeng
    Ming-yao Tian
    Zhuo Ha
    Hui-jun Lu
    Ning-yi Jin
    Archives of Virology, 2022, 167 : 2519 - 2528
  • [50] Efficacy of topical acyclovir monophosphate, acyclovir, or penciclovir in orofacial HSV-1 infections of mice and genital HSV-2 infections of guinea pigs
    Kern, ER
    Palmer, J
    Szczech, G
    Painter, G
    Hostetler, KY
    NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 2000, 19 (1-2): : 501 - 513