Covariation of scleral remodeling and PI3K/Akt signaling pathway in experimental myopia

被引：0

作者：

Xiaomeng Li ^{[1
]}

Xiaojing Liu ^{[2
]}

Yingxin Yu ^{[1
]}

Tuling Li ^{[1
]}

Lijie Guo ^{[1
]}

Guili Hu ^{[3
]}

Huixia Wei ^{[1
]}

Zhaohui Yang ^{[4
]}

Jinpeng Liu ^{[1
]}

Yixian Hao ^{[1
]}

Ruixue Zhang ^{[4
]}

Qiuxin Wu ^{[1
]}

Xuan Liao ^{[4
]}

Dadong Guo ^{[5
]}

Hongsheng Bi ^{[6
]}

机构：

[1] Shandong University of Traditional Chinese Medicine,School of Ophthalmology and Optometry

[2] Wenzhou Medical University,Guangzhou laboratory

[3] Guangzhou Medical University,Department of Ophthalmology of Affiliated Hospital and Medical School of Ophthalmology and Optometry

[4] Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine,Medical College of Optometry and Ophthalmology

[5] North Sichuan Medical College,Medical College of Optometry and Ophthalmology

[6] Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases,undefined

[7] Shandong Academy of Eye Disease Prevention and Therapy,undefined

[8] Shandong University of Traditional Chinese Medicine,undefined

[9] Shandong Academy of Eye Disease Prevention and Therapy,undefined

[10] Shandong University of Traditional Chinese Medicine,undefined

来源：

Scientific Reports | / 15卷 / 1期

关键词：

Experimental myopia; PI3K/Akt signaling pathway; TGF-β1; Collagen; Scleral remodeling;

D O I：

10.1038/s41598-025-97643-7

中图分类号：

学科分类号：

摘要：

The present study aimed to investigate the role of the PI3K/Akt signaling pathway in scleral remodeling in the development of negative lens-induced myopia (LIM). The change of scleral morphology in experimental myopic guinea pigs was observed by transmission electron microscopy, Masson staining, and TUNEL assay, respectively. Meanwhile, the levels of the PI3K/AKT signaling pathway- and scleral remodeling-related molecules in scleral tissues were determined by real-time quantitative PCR (qPCR), enzyme-linked immunosorbent assay (ELISA), immunofluorescence, immunohistochemical staining, and western blot, respectively. We found that 2-week myopic induction can elevate PIK3R3 and AKT2 levels and activate the PI3K/Akt signaling pathway, enhance the expression of E-cadherin and matrix metallopeptidase 2 (MMP2), and decrease the level of transforming growth factor-beta 1 (TGF-β1), tissue inhibitor of matrix metalloproteinase-2 (TIMP2), and collagen (COLI) in the scleral tissue of myopic guinea pigs, thereby leading to scleral remolding. However, 4-week myopic induction could inhibit the PI3K/AKT signaling pathway and induce apoptosis, accompanied by increased MMP2, E-cadherin, and decreased TGF-β1, TIMP2, and COLI. Results reveal that the disturbed PI3K/AKT signaling plays a role in scleral remodeling in the experimental myopia through orchestrating apoptosis.

引用

共 50 条

[1] Elevated retinal fibrosis in experimental myopia is involved in the activation of the PI3K/AKT/ERK signaling pathway
Bao, Bo
Liu, Jinpeng
Li, Tuling
Yang, Zhaohui
Wang, Guimin
Xin, Jizhao
Bi, Hongsheng
Guo, Dadong
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2023, 743
[2] The PI3K/Akt/mTOR signaling pathway
Dennis, P. A.
ANNALS OF ONCOLOGY, 2011, 22 : 19 - 19
[3] The PI3K/AKT/MTOR signaling pathway: The role of PI3K and AKT inhibitors in breast cancer
Huemer F.
Bartsch R.
Gnant M.
Current Breast Cancer Reports, 2014, 6 (2) : 59 - 70
[4] RAS Signaling in the PI3K/AKT/MTOR Pathway
Nussinov, Ruth
Zhang, Mingzhen
Jang, Hyunbum
BIOPHYSICAL JOURNAL, 2020, 118 (03) : 51A - 51A
[5] The effects of Ginsenosides on PI3K/AKT signaling pathway
Soudeh Ghafouri-Fard
Neda Balaei
Hamed Shoorei
Syed Muhammad Farid Hasan
Bashdar Mahmud Hussen
Seyedeh Fahimeh Talebi
Mohammad Taheri
Seyed Abdulmajid Ayatollahi
Molecular Biology Reports, 2022, 49 : 6701 - 6716
[6] PI3k/AKT signaling pathway: Erythropoiesis and beyond
Jafari, Mahjoobeh
Ghadami, Elham
Dadkhah, Tahereh
Akhavan-Niaki, Haleh
JOURNAL OF CELLULAR PHYSIOLOGY, 2019, 234 (03) : 2373 - 2385
[7] Targeting PI3K/AKT signaling pathway in obesity
Savova, Martina S.
Mihaylova, Liliya, V
Tews, Daniel
Wabitsch, Martin
Georgiev, Milen I.
BIOMEDICINE & PHARMACOTHERAPY, 2023, 159
[8] The effects of Ginsenosides on PI3K/AKT signaling pathway
Ghafouri-Fard, Soudeh
Balaei, Neda
Shoorei, Hamed
Hasan, Syed Muhammad Farid
Hussen, Bashdar Mahmud
Talebi, Seyedeh Fahimeh
Taheri, Mohammad
Ayatollahi, Seyed Abdulmajid
MOLECULAR BIOLOGY REPORTS, 2022, 49 (07) : 6701 - 6716
[9] Orientin prevents myocardial remodeling after MI through the PI3K/Akt signaling pathway
Ma, Qiaoli
Han, Bing
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2018, 11 (10): : 10766 - 10773
[10] The role of PI3K/AKT signaling pathway in gallbladder carcinoma
Wu, Zeyu
Yu, Xiao
Zhang, Shuijun
He, Yuting
Guo, Wenzhi
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, 2022, 14 (07): : 4426 - 4442

← 1 2 3 4 5 →