The Antiobesity Mechanisms of Polygonatum cyrtonema Hua Based on Network Analysis and Experimental Validation In Vivo and In Vitro

被引：0

作者：

Xing, Lihua ^{[1
,2
]}

Zhao, Chenchen ^{[1
,2
]}

Xu, Jun ^{[1
,2
]}

Cheng, He ^{[1
,2
]}

Hu, Jiayi ^{[1
,2
]}

Liu, Heng ^{[1
,2
]}

Wang, Mengjin ^{[1
,2
]}

Zhou, An ^{[1
,2
,3
,4
]}

Han, Rongchun ^{[1
,2
,3
,4
]}

Yu, Nianjun ^{[1
,2
,3
,4
]}

机构：

[1] Anhui Univ Chinese Med, Coll Pharm, Hefei 230012, Anhui, Peoples R China

[2] Anhui Acad Chinese Med, Inst Conservat & Dev Tradit Chinese Med Resources, Hefei 230012, Anhui, Peoples R China

[3] Anhui Univ Chinese Med, MOE Anhui Joint Collaborat Innovat Ctr Qual Improv, Hefei 230012, Anhui, Peoples R China

[4] Anhui Univ Chinese Med, Anhui Prov Key Lab Res & Dev Chinese Med, Hefei 230012, Anhui, Peoples R China

来源：

JOURNAL OF FOOD BIOCHEMISTRY | 2025年 / 2025卷 / 01期

关键词：

molecular docking; mTOR signaling pathway; network pharmacology; obesity; Polygonatum cyrtonema Hua; OBESITY; PATHWAY;

D O I：

10.1155/jfbc/3379148

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Obesity is a chronic and complex disease that serves as a precursor to numerous chronic conditions. Its development is linked to gut microbiota and often accompanies inflammation and insulin resistance. The rhizome of Polygonatum cyrtonema Hua is commonly employed in the treatment of metabolic disorders. The mechanisms of P. cyrtonema Hua in treating obesity have not been deeply investigated. This study aimed to explore the protective effect and the possible mechanism of the water extract of P. cyrtonema Hua (PHWE) in high-fat diet-induced obesity in mice. We employed a combination of network pharmacology and molecular docking methods to predict the mechanisms of traditional Chinese medicine in treating obesity, followed by in vivo and in vitro validations. After a series of biological information analyses, 10 potential core targets were identified: AKT1, PPARG, ESR1, STAT3, SIRT1, PPARA, MMP9, FASN, mTOR, and IGF1R. The key pathways mainly include the AMPK signaling pathway, insulin signaling pathway, insulin resistance, and mTOR signaling pathway. In experiments conducted on live mice with obesity, PHWE was found to significantly reduce body weight, IL-6, and IL-1 beta levels in adipose tissue and improve insulin resistance. Based on the characteristics of obesity, the modulation of inflammation and insulin resistance by PHWE in obese mice may be related to gut microbiota. PHWE modulated the mTOR signaling pathway by decreasing the expression of mTOR, Raptor-mTORC1, and pS6K1 proteins while increasing the expression of pIRS1 and pAKT. PHWE could also regulate the protein expression of PPAR gamma and the key target FASN. These findings were further validated in in vitro experiments. Combining network pharmacology with in vivo and in vitro experiments, we investigated the potential mechanisms of PHWE in treating obesity. These findings provide an experimental foundation for the clinical utilization of P. cyrtonema Hua.

引用

页数：16

共 36 条

[1] Conway B., Rene A., Obesity as a Disease: No Lightweight Matter, Obesity Reviews, 5, pp. 145-151, (2004)
[2] Saltiel A.R., Olefsky J.M., Inflammatory Mechanisms Linking Obesity and Metabolic Disease, Journal of Clinical Investigation, 127, pp. 1-4, (2017)
[3] Daryabor G., Kabelitz D., Kalantar K., An Update on Immune Dysregulation in Obesity-Related Insulin Resistance, Scandinavian Journal of Immunology, 89, (2019)
[4] Cai H., Guo Y., Zhao Z., Chen Y., Zhao S., Chen B., Banxia Baizhu Tianma Decoction for Hyperlipidemia: Protocol for a Systematic Review and Meta-Analysis, Medicine, 97, (2018)
[5] Li J., Pang B., Shao D., Jiang C., Hu X., Shi J., Artemisia Sphaerocephala Krasch Polysaccharide Mediates Lipid Metabolism and Metabolic Endotoxaemia in Associated With the Modulation of Gut Microbiota in Diet-Induced Obese Mice, International Journal of Biological Macromolecules, 147, pp. 1008-1017, (2020)
[6] Narayanaswami V., Dwoskin L.P., Obesity: Current and Potential Pharmacotherapeutics and Targets, Pharmacology & Therapeutics, 170, pp. 116-147, (2017)
[7] Hu Y., Yin M., Bai Y., Et al., An Evaluation of Traits, Nutritional, and Medicinal Component Quality of Polygonatum Cyrtonema Hua and P. Sibiricum Red, Frontiers in Plant Science, 13, (2022)
[8] Liu N., Dong Z., Zhu X., Xu H., Zhao Z., Characterization and Protective Effect of Polygonatum Sibiricum Polysaccharide Against Cyclophosphamide-Induced Immunosuppression in Balb/c Mice, International Journal of Biological Macromolecules, 107, pp. 796-802, (2018)
[9] Li L., Thakur K., Liao B.Y., Zhang J.G., Wei Z.J., Antioxidant and Antimicrobial Potential of Polysaccharides Sequentially Extracted From Polygonatum Cyrtonema Hua, International Journal of Biological Macromolecules, 114, pp. 317-323, (2018)
[10] Luan Y., Jiang Y., Huang R., Et al., Polygonati Rhizoma Polysaccharide Prolongs Lifespan and Healthspan in Caenorhabditis Elegans, Molecules, 28, (2023)

← 1 2 3 4 →