Towards Next Generation Protein Sequencing

被引：1

作者：

Yi, Yakun ^{[1
,3
]}

Li, Ziyi ^{[1
,3
]}

Liu, Lei ^{[2
]}

Wu, Hai-Chen ^{[1
,3
]}

机构：

[1] Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China

[2] Xihua Univ, Coll Food & Bioengn, Chengdu 610039, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

CHEMBIOCHEM | 2025年 / 26卷 / 06期

关键词：

protein sequencing; single-molecule analysis; nanopore; ALPHA-HEMOLYSIN; AMINO-ACIDS; PEPTIDES; MASS; REVEALS; DISCRIMINATION; IDENTIFICATION; TRANSLOCATION; SPECTROSCOPY; CAPTURE;

D O I：

10.1002/cbic.202400824

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Understanding the structure and function of proteins is a critical objective in the life sciences. Protein sequencing, a central aspect of this endeavor, was first accomplished through Edman degradation in the 1950s. Since the late 20th century, mass spectrometry has emerged as a prominent method for protein sequencing. In recent years, single-molecule technologies have increasingly been applied to this field, yielding numerous innovative results. Among these, nanopore sensing has proven to be a reliable single-molecule technology, enabling advancements in amino acid recognition, short peptide differentiation, and peptide sequence reading. These developments are set to elevate protein sequencing technology to new heights. The next generation of protein sequencing technologies is anticipated to revolutionize our understanding of molecular mechanisms in biological processes and significantly enhance clinical diagnostics and treatments.

引用

页数：17