Generation of a BEST1 Pr-EGFP reporter human embryonic stem cell line via CRISPR/Cas9 editing

被引：0

作者：

Lu, Shuaiyan ^{[1
,2
,3
]}

Chen, Ming ^{[1
,3
]}

Liu, Xiaoyu ^{[1
,3
]}

Li, Jiahang ^{[1
,3
]}

Liu, Hui ^{[1
,2
,3
]}

Li, Shasha ^{[1
,2
,3
]}

机构：

[1] Wenzhou Med Univ, Eye Hosp, Natl Engn Res Ctr Ophthalmol & Optometry, Wenzhou, Peoples R China

[2] Zhejiang Lab Regenerat Med Vis & Brain Hlth, Oujiang Lab, Wenzhou, Peoples R China

[3] Wenzhou Med Univ, Eye Hosp, State Key Lab Ophthalmol Optometry & Visual Sci, Wenzhou, Peoples R China

来源：

STEM CELL RESEARCH | 2025年 / 82卷

基金：

中国国家自然科学基金;

关键词：

ESRG;

D O I：

10.1016/j.scr.2024.103625

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The retinal pigment epithelium (RPE) cell, located between the neural retina and choriocapillaris, is vital for retinal maintenance and photoreceptor function. Human embryonic stem cells (hESCs) provide a limitless source of RPE cells for transplantation. Using CRISPR/Cas9, we inserted a fusion of the BEST1 promoter (an RPEspecific marker) and the EGFP gene into the AAVS1 locus to track differentiation in hESC-induced RPE (hESC-iRPE). The resulting gene-edited line, WAe009-A-2 maintained a normal karyotype, expressed pluripotency markers, and demonstrated differentiation potential, making it invaluable for RPE development and therapeutic research.

引用

页数：6

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