Microgravity as a Tool to Investigate Cancer Induction in Pleura Mesothelial Cells

被引：0

作者：

Bonetto, Valentina ^{[1
]}

Pagano, Corinna Anais ^{[1
]}

Sabbatini, Maurizio ^{[1
]}

Magnelli, Valeria ^{[1
]}

Donadelli, Massimo ^{[2
]}

Marengo, Emilio ^{[1
]}

Masini, Maria Angela ^{[1
]}

机构：

[1] Univ Piemonte Orientale, Dept Sci & Innovat Technol DISIT, I-15121 Alessandria, Italy

[2] Univ Verona, Dept Neurosci Biomed & Movement Sci DNBM, I-37124 Verona, Italy

来源：

CURRENT ISSUES IN MOLECULAR BIOLOGY | 2024年 / 46卷 / 10期

关键词：

MeT-5A cells; BR95; cells; mesothelioma; focal contacts; connexin-43; NANOG; Fibulin-3; EPITHELIAL-MESENCHYMAL TRANSITION; CDK INHIBITOR P27; DOWN-REGULATION; VINCULIN; ANOIKIS; NANOG; CYTOSKELETON; INITIATION; EXPRESSION; PATHWAYS;

D O I：

10.3390/cimb46100647

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present work shows that the exposure of mesothelial cells to simulated microgravity changes their cytoskeleton and adhesion proteins, leading to a cell switch from normal towards tumoral cells. Immunohistochemical and molecular data were obtained from both MeT-5A exposed to simulated microgravity and BR95 mesothelioma cell lines. Simulated microgravity was found to affect the expression of actin, vinculin, and connexin-43, altering their quantitative and spatial distribution pattern inside the cell. The analysis of the tumoral markers p27, CD44, Fibulin-3, and NANOG and the expression of genes related to cancer transformation such as NANOG, CDH-1, and Zeb-1 showed that the simulated microgravity environment led to expression patterns in MeT-5A cells similar to those observed in BR95 cells. The alteration in both quantitative expression and structural organization of the cytoskeleton and adhesion/communication proteins can thus be considered a pivotal mechanism involved in the cellular shift towards tumoral progression.

引用

页码：10896 / 10912

页数：17

共 57 条

[1] Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT [J].

Aban, C. E. ;

Lombardi, A. ;

Neiman, G. ;

Biani, M. C. ;

La Greca, A. ;

Waisman, A. ;

Moro, L. N. ;

Sevlever, G. ;

Miriuka, S. ;

Luzzani, C. .

SCIENTIFIC REPORTS, 2021, 11 (01)

[2] Mechanisms for Modulating Anoikis Resistance in Cancer and the Relevance of Metabolic Reprogramming [J].

Adeshakin, Funmilayo O. ;

Adeshakin, Adeleye O. ;

Afolabi, Lukman O. ;

Yan, Dehong ;

Zhang, Guizhong ;

Wan, Xiaochun .

FRONTIERS IN ONCOLOGY, 2021, 11

[3]

Allouba Mona H, 2015, Glob Cardiol Sci Pract, V2015, P36, DOI [10.5339/gcsp.2015.36, 10.5339/gcsp.2015.36]

[4] The relationship between cancer and biomechanics [J].

Bao, Liqi ;

Kong, Hongru ;

Ja, Yang ;

Wang, Chengchao ;

Qin, Lei ;

Sun, Hongwei ;

Dai, Shengjie .

FRONTIERS IN ONCOLOGY, 2023, 13

[5] The role of genotoxicity in asbestos-induced mesothelioma: an explanation for the differences in carcinogenic potential among fiber types [J].

Barlow, Christy A. ;

Lievense, Laura ;

Gross, Sherilyn ;

Ronk, Christopher J. ;

Paustenbach, Dennis J. .

INHALATION TOXICOLOGY, 2013, 25 (09) :553-567

[6] Deregulated degradation of the cdk inhibitor p27 and malignant transformation [J].

Bloom, J ;

Pagano, M .

SEMINARS IN CANCER BIOLOGY, 2003, 13 (01) :41-47

[7] Simulated microgravity induces nuclear translocation of Bax and BCL-2 in glial cultured C6 cells [J].

Bonfiglio, Tommaso ;

Biggi, Federico ;

Bassi, Anna Maria ;

Ferrando, Sara ;

Gallus, Lorenzo ;

Loiacono, Fabrizio ;

Ravera, Silvia ;

Rottigni, Marino ;

Scarfi, Sonia ;

Strollo, Felice ;

Vernazza, Stefania ;

Sabbatini, Maurizio ;

Masini, Maria A. .

HELIYON, 2019, 5 (06)

[8] Activation of caspase-8 triggers anoikis in human neuroblastoma cells [J].

Bozzo, Chiarella ;

Sabbatini, Maurizio ;

Tiberio, Rossana ;

Piffanelli, Valentina ;

Santoro, Claudio ;

Cannas, Mario .

NEUROSCIENCE RESEARCH, 2006, 56 (02) :145-153

[9] Spatial distribution and functional significance of activated vinculin in living cells [J].

Chen, H ;

Cohen, DM ;

Choudhury, DM ;

Kioka, N ;

Craig, SW .

JOURNAL OF CELL BIOLOGY, 2005, 169 (03) :459-470

[10] CD44 and its ligand hyaluronan as potential biomarkers in malignant pleural mesothelioma: evidence and perspectives [J].

Cortes-Dericks, Lourdes ;

Schmid, Ralph Alexander .

RESPIRATORY RESEARCH, 2017, 18

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