Integrated Transcriptomic and Metabolomic Analyses Reveal the Importance of the Terpenoid, Fatty Acid, and Flavonoid Pathways in Rice Cell Death and Defense

被引：0

作者：

Bai, Pengfei ^{[1
]}

Liu, Yanfang ^{[1
,2
]}

Gomes-Dias, Laisa ^{[3
,4
,5
]}

Combs-Giroir, Rachel ^{[3
,6
]}

Dai, Shaoxing ^{[7
]}

Choi, Naeyeoung ^{[1
]}

Lin, Yun ^{[3
,4
]}

Bernier, Matthew ^{[8
]}

Hatzakis, Emmanuel ^{[9
]}

Wang, Guo-Liang ^{[1
]}

Blakeslee, Joshua J. ^{[3
,4
,7
]}

机构：

[1] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA

[2] Yunnan Acad Agr Sci, Qual Stand & Testing Technol Res Inst, Kunming 650200, Peoples R China

[3] Ohio State Univ, Dept Hort & Crop Sci, Columbus, OH 43210 USA

[4] Ohio State Univ, Lab Anal Metabolites Plants LAMP, Columbus, OH 43210 USA

[5] Fed Univ Tocantins, Food Sci & Technol Program, BR-77001 Palmas, Brazil

[6] Ohio State Univ, Ctr Appl Plant Sci, Columbus, OH 43210 USA

[7] Kunming Univ Sci & Technol, Inst Primate Translat Med, Kunming 650500, Peoples R China

[8] Ohio State Univ, Campus Chem Instrumentat Ctr CC, Columbus, OH 43210 USA

[9] Ohio State Univ, Dept Food Sci & Technol, Columbus, OH 43210 USA

来源：

PLANTS-BASEL | 2025年 / 14卷 / 05期

基金：

美国食品与农业研究所;

关键词：

rice blast; cell death; metabolomics; transcriptomics; terpenoid; gibberellins; LESION MIMIC MUTANTS; PISUM-SATIVUM-L; PHENYLACETIC ACID; GENETIC DIVERSITY; AUXIN TRANSPORT; EXPRESSION; IMMUNITY; PHYTOALEXIN; RESISTANCE; MECHANISM;

D O I：

10.3390/plants14050665

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Lesion mimic mutants provide unique tools to investigate plant-pathogen interactions, often exhibiting hypersensitive responses in the absence of biotic or abiotic stresses. The overexpression of the S-domain receptor-like kinase gene, SPL11 cell-death suppressor 2 (SDS2), in rice leads to constitutive programmed cell death and enhanced resistance to fungal and bacterial pathogens. However, the mechanisms underlying this broad-spectrum resistance remain unclear. This study integrates transcriptomic and metabolomic analyses of the SDS2-ACT mutant to uncover gene expression and metabolic shifts associated with disease resistance. To identify SDS2-specific physiological changes related to pathogen resistance, leaf tissues from the SDS2-ACT mutant and the Kitkaake WT line were subjected to both transcriptomic and non-targeted metabolic profiling. Transcriptomic analyses identified 1497 differentially expressed genes (DEGs), including up-regulated genes involved in terpenoid and flavonoid biosynthesis, phytohormone signaling, and defense-related pathways (including pathogenesis-related [PR] genes). Metabolomic profiling revealed significant alterations in the accumulation of several compound classes, including putative: terpenoids, phenylpropanoids, phytohormones, fatty acids, and sugars. These changes are likely correlated with the observed cell death and resistance phenotypes in the SDS2-ACT mutant. This study provides an overall landscape of the transcriptomic and metabolomic alterations in a lesion mimic mutant, identifying candidate defense-related genes and metabolites for functional analysis in rice.

引用

页数：22

共 47 条

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