In Vitro Plant Regeneration of Sulla coronaria from Floral Explants as a Biotechnological Tool for Plant Breeding

被引：0

作者：

Auteri, Monica ^{[1
]}

Carra, Angela ^{[2
]}

Di Miceli, Giuseppe ^{[1
]}

Iacuzzi, Nicolo ^{[1
]}

Albano, Alessandro ^{[3
]}

Lala, Nicoletta ^{[1
]}

Motisi, Antonio ^{[2
]}

Catalano, Caterina ^{[2
,4
]}

机构：

[1] Univ Palermo, Dept Agr Food & Forest Sci, Viale Sci,Bldg 4, I-90128 Palermo, PA, Italy

[2] Natl Res Council Italy CNR, Inst Biosci & BioResources IBBR, Via Ugo La Malfa 153, I-90146 Palermo, PA, Italy

[3] Univ Palermo, Dept Econ Business & Stat, Viale Sci,Bldg 13, I-90128 Palermo, PA, Italy

[4] Natl Res Council Italy CNR, Inst Agr Biol & Biotechnol IBBA, Via Alfonso Corti 12, I-20133 Milan, MI, Italy

来源：

AGRONOMY-BASEL | 2024年 / 14卷 / 11期

关键词：

forage crop; factors affecting regeneration; biotypes; PGR combinations; light conditions; SOMATIC EMBRYOGENESIS; HEDYSARUM-CORONARIUM; CONDENSED TANNINS; WOOL GROWTH; CULTURE; FORAGE; L; PARAMETERS; CULTIVARS; GENOTYPE;

D O I：

10.3390/agronomy14112667

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Sulla coronaria L. Medik., a biennial forage legume typical of the Mediterranean basin, plays a significant role in foraging systems due to its high nutritional value, ability to increase ruminant live weight, and potential to reduce methane emissions. However, its allogamous nature complicates genetic improvement and the development of new varieties with desired traits. Given these challenges, this study aims to develop, for the first time, a successful protocol for the in vitro meristematic shoot regeneration of S. coronaria. The experiment utilizes four different flower explants (anther with filament, ovary, petals, and whole immature flower) collected from twenty distinct S. coronaria biotypes with three plant growth regulator (PGR) combinations and under both light and dark conditions. In terms of the regeneration response, the key factors appear to be the combinations of PGRs and the type of explant used. The interactions between all the factors do not seem to be significant.

引用

页数：11

共 56 条

[31]

Jiménez VM, 2006, PLANT CELL MONOGR, V2, P103, DOI 10.1007/7089_034

[32] Somatic embryogenesis in leguminous plants [J].

Lakshmanan, P ;

Taji, A .

PLANT BIOLOGY, 2000, 2 (02) :136-148

[33] Influence of growth regulators on somatic embryogenesis in sesame [J].

Jeya Mary R. ;

Jayabalan N. .

Plant Cell, Tissue and Organ Culture, 1997, 49 (1) :67-70

[34]

McCulloch CharlesE., 2000, Generalized, linear, and mixed models

[35] Cover crops for saline soils [J].

Mitchell, JP ;

Thomsen, CD ;

Graves, WL ;

Shennan, C .

JOURNAL OF AGRONOMY AND CROP SCIENCE-ZEITSCHRIFT FUR ACKER UND PFLANZENBAU, 1999, 183 (03) :167-178

[36] A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].

MURASHIGE, T ;

SKOOG, F .

PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497

[37] Agroecological impact of an in vitro biotechnology approach of embryo development and seed filling in legumes [J].

Ochatt, Sergio J. .

AGRONOMY FOR SUSTAINABLE DEVELOPMENT, 2015, 35 (02) :535-552

[38] Studies on Improving the Efficiency of Somatic Embryogenesis in Grapevine (Vitis vinifera L.) and Optimising Ethyl Methanesulfonate Treatment for Mutation Induction [J].

Pathirana, Ranjith ;

Carimi, Francesco .

PLANTS-BASEL, 2023, 12 (24)

[39]

Piluzza G., 2000, Cahiers Options Mediterraneennes, V45, P199

[40] Factors affecting maintenance, proliferation, and germination of secondary somatic embryos of Eucalyptus globulus Labill [J].

Pinto, Gloria ;

Park, Yill-Sung ;

Silva, Sonia ;

Neves, Lucinda ;

Araujo, Clara ;

Santos, Conceicao .

PLANT CELL TISSUE AND ORGAN CULTURE, 2008, 95 (01) :69-78

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