Carbonic anhydrase 2 mediates anti-obesity effects of black tea as thermogenic activator

被引：3

作者：

Ma, Peng ^{[1
,2
]}

Xiao, Jie ^{[3
]}

Hou, Biyu ^{[1
,2
]}

He, Ping ^{[4
]}

Yang, Xinyu ^{[5
]}

Wang, Yisa ^{[1
,2
,6
]}

Wang, Zijing ^{[1
,2
]}

Xu, Tianshu ^{[1
,2
]}

Yang, Xiuying ^{[1
,2
]}

Zhu, Xuan ^{[7
]}

Xiang, Shasha ^{[7
]}

Li, Song ^{[8
]}

Du, Guanhua ^{[1
,2
]}

Ying, Jian ^{[2
,3
,9
]}

Qiang, Guifen ^{[1
,2
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Beijing 100050, Peoples R China

[2] Beijing Key Lab Drug Target & Screening Res, Beijing 100050, Peoples R China

[3] COFCO Nutr & Hlth Res Inst, Beijing 102209, Peoples R China

[4] Sun Yat Sen Univ, Affiliated Hosp 8, Dept Pharm, Shenzhen 518000, Peoples R China

[5] Univ Manchester, Fac Biol Med & Hlth Sci, Manchester M13 9PL, England

[6] Changchun Univ Chinese Med, Changchun 130117, Peoples R China

[7] Zhejiang Gongshang Univ, Sch Food Sci & Biotechnol, Hangzhou 310018, Peoples R China

[8] China Tea Technol Beijing Co Ltd, Beijing 102209, Peoples R China

[9] Beijing Key Lab Nutr & Hlth & Food Safety, Beijing 102209, Peoples R China

来源：

FOOD SCIENCE AND HUMAN WELLNESS | 2024年 / 13卷 / 05期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

Obesity; Black tea; Thermogenesis; Browning; Adipose tissue; WHITE ADIPOSE-TISSUE; CARDIOMETABOLIC RISK; GREEN TEA; OBESITY; FAT; EXPRESSION; PATHWAY;

D O I：

10.26599/FSHW.2022.9250236

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Obesity is a metabolic disorder due to over-accumulation of adipose tissue and ultimately becomes a "disease". Brown adipose tissue (BAT) thermogenesis and white adipose tissue (WAT) browning emerge as a potential strategy of anti-obesity by dissipating energy as heat. However, drugs based on adipose tissue thermogenesis have not been successfully approved yet. In current study, we found that black tea extract (BTE) obtained by patentauthorized manufacturing process prevented body weight gain as novel thermogenic activator with reduction of adiposity, improvement of adipose distribution, and glucose metabolism improvement in diet-induced obesity mice. Mechanismly, anti-obesity effect of BTE depends on promoting BAT thermogenesis and WAT browning with upregulation of uncoupling protein 1 (UCP1), especially visceral adipose tissue (VAT) with browning resistance. Specifically, utilizing in silico approach of network pharmacology and molecular docking, we identified carbonic anhydrase 2 (CA2) in nitrogen metabolism as anti-obesity target of BTE and further elucidated that protein kinase B (AKT) signaling pathway linked CA2 and UCP1. Meanwhile gut microbiota regulation may prompt the CA2-dependent thermogenesis activation. Our findings demonstrated anti-obesity effect of BTE as thermogenic activator through CA2-mediated BAT thermogenesis and WAT browning via CA2-AKT-UCP1 signaling pathway, which could be developed as promising anti-obesity agent with good safety and efficacy. (c) 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

引用

页码：2917 / 2936

页数：20