Comparative analysis of anticoagulant influence on PMI estimation based on porcine blood metabolomics profile measured using GC-MS

被引:0
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作者
Mojsak, Patrycja [1 ]
Samczuk, Paulina [1 ,2 ]
Klimaszewska, Paulina [1 ]
Burdukiewicz, Michal [1 ,3 ]
Chilimoniuk, Jaroslaw [1 ]
Grzesiak, Krystyna [1 ,4 ]
Pietrowska, Karolina [1 ]
Ciborowska, Justyna [5 ]
Niemcunowicz-Janica, Anna [6 ]
Kretowski, Adam [1 ,7 ]
Ciborowski, Michal [1 ,8 ]
Szeremeta, Michal [6 ]
机构
[1] Med Univ Bialystok, Clin Res Ctr, Metabol & Prote Lab, Bialystok, Poland
[2] Cent Forens Lab Police, Dept Genet Res, Warsaw, Poland
[3] Autonomous Univ Barcelona, Inst Biotechnol & Biomed, Cerdanyola Del Valles, Spain
[4] Univ Wroclaw, Fac Math & Comp Sci, Wroclaw, Poland
[5] Forens Lab Voivodeship Police Headquarters Bialyst, Chem Res Lab, Bialystok, Poland
[6] Med Univ Bialystok, Dept Forens Med, Bialystok, Poland
[7] Med Univ Bialystok, Dept Endocrinol Diabetol & Internal Med, Bialystok, Poland
[8] Med Univ Bialystok, Dept Med Biochem, Bialystok, Poland
关键词
post-mortem interval (PMI); animal model; blood biomarkers; metabolomics; GC-MS; POSTMORTEM INTERVAL ESTIMATION; VITREOUS-HUMOR; TIME; RATS; BIOMARKERS; LEVEL; DEATH;
D O I
10.3389/fmolb.2024.1400622
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Introduction Accurate post-mortem interval (PMI) estimation is essential in forensic investigations. Although various methods for PMI determination have been developed, only an approximate estimation is still achievable, and an accurate PMI indication is still challenging. Therefore, in this study, we employed gas chromatography-mass spectrometry (GC-MS)-based metabolomics to assess post-mortem changes in porcine blood samples collected with and without the addition of anticoagulant (EDTA). Our study aimed to identify metabolites dependent on the EDTA addition and time (taking into account the biodiversity of the studied organism) and those that are time-dependent but resistant to the addition of an anticoagulant.Methods The experiment was performed on blood samples collected from 16 animals (domestic pig, breed: Polish Large White), 8 with and 8 without EDTA addition. The moment of death (time 0) and 15 additional time points (from 3 to 168 h after death) were selected to examine changes in metabolites' levels in specific time intervals. We employed linear mixed models to study the relationship between metabolite intensities, time and presence of EDTA while accounting for the effect of individual pigs.Results and Discussion We confirmed that the intensity of 16 metabolites (mainly amino acids) significantly depends on PMI and the presence of EDTA. However, the intensity of the ideal biomarker(s) for PMI estimation should be determined only by the time after death and not by external factors such as the presence of the anticoagulant agent. Thus, we identified 41 metabolites with time-dependent intensities that were not susceptible to EDTA presence. Finally, we assessed the performance of these metabolites in a PMI predictive model. Citraconic acid yielded one of the lowest errors in general PMI estimation (32.82 h). Moreover, similar errors were observed for samples with and without EDTA (33.32 h and 32.34 h, respectively). Although the small sample size and information leak in predictive modelling prevent drawing definite conclusions, citraconic acid shows potential as a robust PMI estimator.
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页数:10
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