Effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice

被引：0
作者：
Ma, Jing [1 ]
Gong, Zheng [1 ]
Kang, Meiling [1 ]
Tian, Zhongjing [1 ]
You, Liping [2 ]
Ding, Chengshi [1 ]
机构：
[1] Zaozhuang Univ, Coll Life Sci, Zaozhuang, Peoples R China
[2] Zibo Cent Hosp, Dept Clin Lab, Zibo, Peoples R China
来源：
FRONTIERS IN MICROBIOLOGY | 2025年 / 15卷
关键词：
multidrug-resistant Escherichia coli; multi-antibiotic combination; intestinal microbiota; high-throughput sequencing; antibiotics; ANTIMICROBIAL RESISTANCE; GENES; HEALTH;
D O I：
10.3389/fmicb.2024.1504396
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Multidrug-resistant bacteria are a clinical and an epidemiological challenge. However, the effects of multidrug-resistant bacteria and multi-antibiotic combination on gut microbiota are unclear. In this study, the effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice have been observed by high-throughput sequencing. Resistant Escherichia coli (RP4) and 0.5, 1, and 2 mg/L antibiotics (Amp, Km, and Tet multi-antibiotic combination) could decrease the number of specific operational taxonomic units from 223 in the normal saline control group to 178 in the antibiotic-resistant bacteria group and 34 in the antibiotic group, and antibiotics are the biggest influencing factor. Multidrug-resistant bacteria and multi-antibiotic combination could affect the function of intestinal microbiota, and the effect of multidrug-resistant bacteria was similar to that of multi-antibiotic combination. Small intestine is the main colonization site of antibiotic-resistant bacteria, and Proteobacteria and Bacteroidetes are the major antibiotic-resistance acquired bacteria as determined by transmission electron microscopy and agarose plate screening culture.
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页数：10
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