DETECTION OF Staphylococcus aureus VIA APTAMER-GATED MCM-41 NANOPARTICLES

Objective: Aptamer-gated nanoparticles are a possible way to find Staphylococcus aureus because they are very specific, sensitive, and quick to detect. Materials and Methods: MCM-41 nanoparticles were characterized via DLS, SEM, and FTIR techniques. Reference strains S. aureus, S. epidermidis, and Escherichia coli were used. After the synthesis of the fluorescence-loaded silica-coated MCM-41 nanoparticles, fluorescence release experiments were performed via a dialysis membrane. Results: The particle size of the MCM-41 nanoparticles was determined to be 192 +/- 1.782 nm. BET analysis revealed that each MCM-41 particle had a specific surface area of 1019.37 m2/g, pores that were 2.42 nm wide, and the ability to hold 0.99 cm3/g of material. The MCM-41 nanoparticles were nanosized, had a narrow size distribution, and were smooth, amorphous and spherical in shape. Amino group-functionalized MCM-41 nanoparticles via the APTES reaction. FT-IR analysis was performed to determine the correct conjugation. After the addition of amino acids, typical bands at 690 and 1460 nm appeared. These bands correspond to N-H bending vibrations and N-H asymmetric bending vibrations, respectively. The fluorescein-loaded silica particles conjugated with the aptamer and the target bacterium S. aureus had the maximum release. Furthermore, approximately 70% fluorescein release occurred within 6 hours. It was possible to quickly and accurately find S. aureus at detection limits as low as 164 CFU/mL in PBS. Conclusion: The proposed biosensor has many benefits, such as quick response times, high sensitivity, and specificity for S. aureus detection. Future studies will likely concentrate on increasing the sensitivity of these technologies, decreasing detection times, and broadening their range of applications.