Exploring the Drivers of Ocelot Population Density: A Systematic Review and Meta-Analysis of Methodological and Ecological Influences

BackgroundCamera trapping associated with capture-recapture models is commonly used to estimate wild felid population density, relying on three main frameworks: spatially explicit capture-recapture (SECR) and two non-spatial approaches (CR-MMDM and CR-hMMDM). Methodological differences, inappropriate sampling designs, and/or insufficient data explain some estimate variability, but the biological factors underpinning this remain undetermined. Prey availability, habitat suitability, and body size may all interact and influence carnivoran population size and density.AimsWe aimed to (1) survey ocelot population density data and summarise information on study designs, methodological approaches, and results, (2) evaluate the relationships between them, (3) disentangle methodological and ecological effects on population density estimates, and (4) provide guidance to improve study design and reporting.Materials & MethodsOur systematic review discovered 51 studies reporting 228 ocelot population density estimates from 65 sites across 13 countries. We collated ocelot body mass data (BM) and used forest canopy height (GFCH) as a surrogate for habitat suitability, as well as gross primary productivity seasonality (GPP variation) as a proxy for prey availability. Using a meta-analytical framework, we created models to (1) determine mean ocelot population density in the Neotropics and to assess the effects of (2) methodological and (3) ecological variables on population density estimates.ResultsMean population density was 20.3/100 km2, with significant differences among methods. SECR and CR-MMDM yielded comparable estimates (16.6/100 km2 and 18.9/100 km2, respectively), while CR-hMMDM produced higher estimates (27.3/100 km2). We found significant positive and negative effects of GFCH and BM, respectively, and a marginally significant negative effect of GPP variation on estimates.DiscussionOcelots thrive in forests with higher canopies, but their population density is limited by local habitat seasonality. Morphological differences further influence variation, with small-bodied populations attaining higher population densities under similar ecological conditions.ConclusionBased on our findings, we provide guidelines to enhance the accuracy and standardization of study designs, methodological approaches, and general reporting. Improving these aspects will strengthen the comparability and reliability of ocelot population density estimates.