Enhancing recombinant antibody yield in Chinese hamster ovary cells

被引：2
作者：
Yang, Chee-Hing [1 ]
Li, Hui-Chun [2 ]
Lo, Shih-Yen [3 ,4 ]
机构：
[1] Tzu Chi Univ, Sch Med, Dept Microbiol & Immunol, Hualien, Taiwan
[2] Tzu Chi Univ, Sch Med, Dept Biochem, Hualien, Taiwan
[3] Tzu Chi Univ, Dept Lab Med & Biotechnol, 701,Sect 3,Chung Yang Rd, Hualien, Taiwan
[4] Hualien Tzu Chi Hosp, Dept Lab Med, Buddhist Tzu Chi Med, Hualien, Taiwan
来源：
TZU CHI MEDICAL JOURNAL | 2024年 / 36卷 / 03期
关键词：
Cell engineering; Chinese hamster ovary cells; Plasmid construction; Recombinant antibody; MONOCLONAL-ANTIBODY; GENE-EXPRESSION; MESSENGER-RNA; CHO-CELLS; DISULFIDE-ISOMERASE; PROTEIN-PRODUCTION; VECTOR DESIGN; CULTURE; LINES; GENERATION;
D O I：
10.4103/tcmj.tcmj_315_23
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
A range of recombinant monoclonal antibodies (rMAbs) have found application in treating diverse diseases, spanning various cancers and immune system disorders. Chinese hamster ovary (CHO) cells have emerged as the predominant choice for producing these rMAbs due to their robustness, ease of transfection, and capacity for posttranslational modifications akin to those in human cells. Transient transfection and/or stable expression could be conducted to express rMAbs in CHO cells. To bolster the yield of rMAbs in CHO cells, a multitude of approaches have been developed, encompassing vector optimization, medium formulation, cultivation parameters, and cell engineering. This review succinctly outlines these methodologies when also addressing challenges encountered in the production process, such as issues with aggregation and fucosylation.
引用
收藏
页码：240 / 250
页数：11
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