Cultivation Strategies to Improve Chlamydomonas reinhardtii Growth and Recombinant Mcherry Expression

被引:0
作者
Pessoa, Jassiara da Silva [1 ]
da Silva, Bruno Guzzo [1 ]
de Franca Jr, Ednilson Donisete [1 ]
da Silva Filho, Isac Jose [1 ]
Molino, Joao Vitor Dutra
de Carvalho, Joao Carlos Monteiro [2 ]
Ferreira-Camargo, Livia Seno [1 ]
机构
[1] Fed Univ ABC, Ctr Nat & Human Sci, Santo Andre, SP, Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci, Sao Paulo, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
CCRD; Chlamydomonas reinhardtii; fluorescent protein; growth optimization; mCherry; transgenic microalga; FLUORESCENT PROTEINS; SYSTEM;
D O I
10.1002/jobm.70006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Chlamydomonas reinhardtii is a promising model microalga for recombinant molecules production. Nonetheless, low yield is a challenge for its industrial use. This work investigated the influence of ammonium chloride (NH4Cl) concentration and temperature on the growth of transgenic C. reinhardtii expressing the fluorescent protein mCherry on a laboratory scale. A Central Composite Rotatable Design was used to establish the cultivation conditions. NH4Cl concentrations ranging from 400 to 647.49 mg/L and temperatures between 25 degrees C and 32.1 degrees C resulted in maximum values of cell concentration and mCherry fluorescence. Lower temperatures (15 degrees C-17 degrees C) were found to be more suitable for the accumulation of total soluble proteins. These results demonstrate that cultivation conditions can positively affect C. reinhardtii growth, with a range of conditions that can be used. Unlike genetic approaches, this study provides a solution to enhance both growth and recombinant protein expression in C. reinhardtii. These findings pave the way for scaling up the use of C. reinhardtii as a biofactory in industry and can be applied to other microalgal systems.
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页数:8
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