Mesenchymal stem cells versus mesenchymal stem cells-derived exosomes as potential autophagy pathway modulators in a diabetic model

被引：0

作者：

Meligy, Fatma Y. ^{[1
,2
]}

Mohammed, Hanan Sharaf El-Deen ^{[3
]}

Elghait, Amal T. Abou ^{[2
,4
]}

Mohamed, Heba K. ^{[5
,6
]}

Ashry, Israa El-Sayed Mohamed ^{[7
]}

Sayed, Ayat Abdel-Rahman ^{[8
,9
]}

Hussein, Ola A. ^{[2
]}

Salman, Ahmed ^{[10
,11
]}

Atia, Tarek ^{[12
]}

Mohamed, Abir S. ^{[13
]}

Behnsawy, Nour H. ^{[14
,15
]}

Gaber, Safy Salah ^{[16
]}

Sakr, Hader I. ^{[17
,18
]}

Ahmed, Salwa Fares ^{[2
,19
]}

机构：

[1] Univ Petra, Fac Dent, Dept Restorat Dent & Basic Med Sci, Amman, Jordan

[2] Assiut Univ, Fac Med, Histol & Cell Biol Dept, Assiut, Egypt

[3] Assiut Univ, Fac Med, Internal Med Dept, Crit Care Unit, Assiut, Egypt

[4] Sphinx Univ, Histol & Cell Biol Dept, Assiut, Egypt

[5] Assiut Univ, Anat & Embryol Dept, Fac Med, Assiut, Egypt

[6] Sphinx Univ, Assiut, Assiut, Egypt

[7] Assiut Univ, Fac Med, Pharmacol Dept, Assiut, Egypt

[8] Assiut Univ, Fac Med, Med Biochem Dept, Assiut, Egypt

[9] Sphinx Univ, Dept Biochem, Assiut, Egypt

[10] Univ Jordan, Fac Med, Dept Anat & Histol, Amman, Jordan

[11] Menoufia Univ, Fac Med, Dept Anat & Embryol, Menoufia, Egypt

[12] Prince Sattam Bin Abdulaziz Univ, Coll Appl Med Sci, Dept Med Lab, Al Kharj, Saudi Arabia

[13] Jazan Univ, Coll Nursing & Hlth Sci, Dept Publ Hlth, Jazan, Saudi Arabia

[14] Assiut Univ, Fac Med, Assiut, Egypt

[15] Univ Uberaba UNIUBE, Cairo, Brazil

[16] Beni Suef Univ, Fac Med, Dept Med Physiol, Bani Suwayf, Egypt

[17] Cairo Univ, Fac Med, Dept Med Physiol, Cairo, Egypt

[18] Batterjee Med Coll, Dept Med Physiol, Gen Med Practice Program, Jeddah 21442, Saudi Arabia

[19] Univ Tabuk, Fac Med, Anat Dept, Tabuk, Saudi Arabia

来源：

ADVANCES IN MEDICAL SCIENCES | 2025年 / 70卷 / 01期

关键词：

Diabetes mellitus; Mesenchymal stem cells; Exosome; Autophagy; STROMAL CELLS; EXTRACELLULAR VESICLES; BONE-MARROW; TISSUE; RECOVERY; OBESITY; STROKE; REPAIR;

D O I：

10.1016/j.advms.2025.02.004

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Purpose: This work compared the potential effects of bone marrow mesenchymal stem cells (BM-MSCs) with BMMSCs-derived exosomes against impaired autophagy in streptozotocin (STZ)-induced diabetic rats. Materials and methods: Three days after STZ injection, a single dose of (3 x 10<^>6) BM- MSCs or BM-MSCs-derived exosomes (80 mu g/rat) was administered to evaluate their effects against nondiabetic and diabetic control rats. We assessed pancreatic structure via light and electron microscopy and evaluated its staining for insulin and the autophagy marker P62 immunohistochemically. Moreover, autophagy marker LC3 gene expression was examined by PCR. Results: Both BM-MSCs and BM-MSCs derived exosomes showed histological restoration of pancreatic tissues. Both treatments markedly increased the amount of insulin and significantly decreased the autophagy markers P62 and LC3. Conclusion: Our findings suggest that both BM-MSCs and BM-MSCs-derived exosomes provides a potential alternative to modulate diabetes mellitus.

引用

页码：152 / 165

页数：14

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