Gold nanoparticles synthesized from soil-derived Streptomyces sp. ASM19: characterization, antimicrobial, anticancer potency targeted G2/M phase cell-cycle arrest, and in silico studies

被引：0

作者：

Aati, Sultan ^{[1
]}

Aati, Hanan Y. ^{[2
]}

Hamed, Ahmed A. ^{[3
]}

El-Shamy, Sherine ^{[4
]}

Aati, Shahad H. ^{[5
]}

Abdelmohsen, Usama Ramadan ^{[6
,7
]}

Bringmann, Gerhard ^{[8
]}

Taha, Mostafa N. ^{[9
]}

Hassan, Hossam M. ^{[10
,11
]}

Bahr, Hebatallah S. ^{[12
]}

机构：

[1] King Saud Univ, Coll Appl Med Sci, Dent Hlth Dept, Riyadh, Saudi Arabia

[2] King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh 11495, Saudi Arabia

[3] Natl Res Ctr, Microbial Chem Dept, 33 El Buhouth St, Giza, Egypt

[4] Modern Univ Technol & Informat, Fac Pharm, Pharmacognosy Dept, Cairo, Egypt

[5] King Saud Univ, Coll Dent, Riyadh, Saudi Arabia

[6] Deraya Univ, Deraya Ctr Sci Res, New Minia 61111, Egypt

[7] Minia Univ, Fac Pharm, Pharmacognosy Dept, Al Minya, Egypt

[8] Univ Wurzburg, Inst Organ Chem, D-97074 Wurzburg, Germany

[9] Nahda Univ, Fac Pharm, Dept Microbiol & Immunol, Bani Suwayf 62513, Egypt

[10] Kut Univ Coll, Dept Pharm, Alkut 52001, Wasit, Iraq

[11] Beni Suef Univ, Fac Pharm, Dept Pharmacognosy, Bani Suwayf 62514, Egypt

[12] Nahda Univ, Fac Pharm, Dept Pharmacognosy, Bani Suwayf 62513, Egypt

来源：

RSC ADVANCES | 2025年 / 15卷 / 05期

关键词：

SILVER NANOPARTICLES; MARINE; BIOSYNTHESIS; DERIVATIVES; INHIBITOR; ANTIBIOTICS; CULTURES; ACID;

D O I：

10.1039/d4ra07608g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Gold nanoparticles (Au) have attracted considerable attention in the field of biomedicine in recent years. The present work was designed to investigate gold nanoparticles obtained using a soil-associated actinomycetes, Streptomyces sp. ASM19. This microorganism was isolated and identified using DNA sequencing. The chemical profile of the Streptomyces sp. ASM19 extract was analyzed using LC-HRES-MS. Streptomyces sp. ASM19 extract was utilized to synthesize actinomycetes-based gold nanoparticles (Ac-AuNPs), which were analyzed using ultraviolet-visible (UV-vis) spectrophotometry, Fourier transform infrared (FTIR) spectroscopy, and atomic force microscopy (AFM). In addition, the antibacterial, and anti-biofilm, as well as, the anti-proliferative properties of Ac-AuNPs against seven cancer lines were investigated. LC-HRES-MS analysis traced a total of 111 peaks, 14 of them are key peaks belonging to the chemical classes, alkaloids, steroids, and polyketides. Analysis of the synthesized Ac-AuNPs revealed that they exhibited a wine-red color and a plasmon band appeared at 540 nm, confirming the formation of the Ac-AuNPs. Further, FTIR confirmed various functional groups present in Ac-AuNPs. The crude extract of Streptomyces sp. ASM19 demonstrated consistent antibacterial activity in contrast to Ac-AuNPs. The anti-proliferative properties of Ac-AuNPs demonstrated encouraging anticancer properties against SCC9 and SCC25 cell lines with IC50 values of 3.77 and 1.56 mu g mL-1. Furthermore, Ac-AuNPs had total apoptotic percentages of 26.37% (SCC9) and 32.08% (SCC25), which are around 25 times higher than the control (0.95%). Additionally, it caused a notable G2/M phase cell-cycle arrest. On the other hand, molecular docking study carried out for the annotated compounds; tomaymycin (8) and nocapyrone P (5), showed considerable binding affinities compared to the co-crystallized inhibitor (fisetin) against the cyclin-dependent kinase 6 active site. Overall, the present study could be useful for nano drug delivery and may be applied for clinical studies in the future.

引用

页码：3954 / 3968

页数：15