Combining isolated G-quadruplexes with CHA-3WJ/DNA: A triple signal amplification colorimetric biosensor for detecting Escherichia coli O157: H7

被引：2
作者：
Pang, Lidong [1 ]
Li, Runze [1 ]
Su, Qunchao [1 ]
Li, Shihang [1 ]
Zhao, Qianyu [1 ]
Zhang, Wei [1 ]
Yang, Xinyan [1 ]
Jiang, Yujun [1 ,2 ]
机构：
[1] Northeast Agr Univ, Coll Food Sci, Key Lab Dairy Sci, Minist Educ, Harbin 150030, Peoples R China
[2] Food Lab Zhongyuan, Luohe 462300, Henan, Peoples R China
来源：
FOOD BIOSCIENCE | 2024年 / 62卷
基金：
中国国家自然科学基金;
关键词：
E. coli O157; H7; Colorimetric; Detection; Catalytic hairpin assembly; G-quadruplex; Aptamer-functionalized magnetic beads;
D O I：
10.1016/j.fbio.2024.105303
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
In this study, a colorimetric biosensor was developed based on the use of aptamer-functionalized magnetic beads and catalytic hairpin assembly (CHA), in conjunction with a three-way junction DNA (3WJ/DNA) and isolated Gquadruplex/hemin DNAzyme triple signal amplification. This approach enabled the label-free, visual and sensitive detection of Escherichia coli O157: H7. The target bacterium binds to the aptamer, which releases complementary DNA, thereby triggering the CHA to produce 3WJ/DNA. The three ends of 3WJ/DNA can each form a G-quadruplex/DNAzyme, thereby catalyzing the 3,3 ',5,5 '-tetramethylbenzidine (TMB)-H2O2 system to turn blue. Notably, we provide a triple signal amplification method of G-quadruplex/hemin DNAzyme based on the triple end of CHA-3WJ/DNA by cleverly screening and designing an isolated G-quadruplex, significantly improving the sensitivity. The proposed method's limit of detection (LOD) was 8 CFU/mL, exhibiting good linearity within the range of 10 degrees-108 CFU/mL. Impressively, the method successfully detected E. coli O157: H7 in ultrahightemperature-sterilized, pasteurized and raw milk, exhibiting good stability and reproducibility.
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页数：9
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