Inhibition of Aflatoxin B1 Production by Procyanidins Present in Annona muricata and Uncaria tomentosa Aqueous Extracts

被引：1

作者：

Cadenillas, Laura F. ^{[1
]}

Billerach, Guillaume ^{[1
]}

Hernandez, Christopher ^{[1
]}

Durrieu, Vanessa ^{[1
]}

Bailly, Jean-Denis ^{[1
,2
]}

机构：

[1] Univ Toulouse, Lab Chim Agroind LCA, INRAe, INPT, 4 allee Emile Monso, F-31030 Toulouse, France

[2] Ecole Natl Vet Toulouse, 23 Chemin Capelles, F-31076 Toulouse, France

来源：

TOXINS | 2024年 / 16卷 / 11期

关键词：

Aflatoxin B-1; Annona muricata; Uncaria tomentosa; antioxidant activity; procyanidins; ANTIOXIDANT ACTIVITY; ASPERGILLUS-FLAVUS; OXIDATIVE STRESS; ESSENTIAL OIL; FUNGAL; BIOSYNTHESIS; POLYPHENOL; CAPACITY; EFFICACY; GROWTH;

D O I：

10.3390/toxins16110454

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Aflatoxin B-1 (AFB(1)), primarily produced by Aspergillus flavus and A. parasiticus, is the most dangerous mycotoxin for humans and contaminates a variety of crops. To limit fungal growth and aflatoxin production in food and feed, research has been increasingly focusing on alternatives to pesticides. Studies show that some aqueous plant extracts with strong antioxidant properties could significantly impact AFB(1) production, representing an eco-friendly and sustainable method to protect crops. The present study demonstrates that aqueous extracts of Anonna muricata (AM) and Uncaria tomentosa (UT) inhibit AFB(1) synthesis in a dose-dependent manner with a half-maximal inhibitory concentration of 0.25 and 0.28 mg dry matter per milliliter of culture medium, respectively. This effect correlates with the presence of polyphenols and, more precisely, with condensed tannins. It is also related to the subsequent antioxidant activity of both extracts. A bio-guided fractionation followed by high-performance liquid chromatography and mass spectrometry analysis of the active fractions identifies procyanidins and, more precisely, catechin (5.3% w/w for AM and 5.4% w/w for UT) and epicatechin (10.6% w/w for AM and 25.7% w/w for UT) as the major components in both extracts. The analysis of how pure standards of these molecules affect AFB(1) production demonstrates that catechin plays an essential role in the inhibition observed for both plant extracts, since the pure standard inhibits 45% of AFB(1) synthesis at a concentration close to that of the extracts.

引用

页数：15

共 58 条

[11] Ribeiro L.P., Domingues V.C., Goncalves G.L.P., Fernandes J.B., Gloria E.M., Vendramim J.D., Essential Oil from Duguetia lanceolata St.-Hil. (Annonaceae): Suppression of Spoilers of Stored-Grain, Food Biosci, 36, (2020)
[12] Loi M., Paciolla C., Logrieco A.F., Mule G., Plant Bioactive Compounds in Pre- and Postharvest Management for Aflatoxins Reduction, Front. Microbiol, 11, (2020)
[13] Caceres I., Al Khoury A., El Khoury R., Lorber S., Oswald I.P., El Khoury A., Atoui A., Puel O., Bailly J.-D., Aflatoxin Biosynthesis and Genetic Regulation: A Review, Toxins, 12, (2020)
[14] Peng K., Jin L., Niu Y.D., Huang Q., McAllister T.A., Yang H.E., Denise H., Xu Z., Acharya S., Wang S., Et al., Condensed Tannins Affect Bacterial and Fungal Microbiomes and Mycotoxin Production during Ensiling and upon Aerobic Exposure, Appl. Environ. Microbiol, 84, (2018)
[15] Hernandez C., Cadenillas L., Maghubi A.E., Caceres I., Durrieu V., Mathieu C., Bailly J.-D., Mimosa tenuiflora Aqueous Extract: Role of Condensed Tannins in Anti-Aflatoxin B1 Activity in Aspergillus flavus, Toxins, 13, (2021)
[16] Wahab S.M.A., Jantan I., Haque M.A., Arshad L., Exploring the Leaves of Annona muricata L. as a Source of Potential Anti-Inflammatory and Anticancer Agents, Front. Pharmacol, 9, (2018)
[17] Adewole S., Ojewole J., Protective Effects of Annona muricata Linn. (Annonaceae) Leaf Aqueous Extract on Serum Lipid Profiles and Oxidative Stress in Hepatocytes of Streptozotocin-Treated Diabetic Rats, Afr. J. Tradit. Complement. Altern. Med, 6, pp. 30-41, (2009)
[18] Hajdu Z., Hohmann J., An Ethnopharmacological Survey of the Traditional Medicine Utilized in the Community of Porvenir, Bajo Paraguá Indian Reservation, Bolivia, J. Ethnopharmacol, 139, pp. 838-857, (2012)
[19] Balderrama-Carmona A.P., Silva-Beltran N.P., Galvez-Ruiz J.-C., Ruiz-Cruz S., Chaidez-Quiroz C., Moran-Palacio E.F., Antiviral, Antioxidant, and Antihemolytic Effect of Annona muricata L. Leaves Extracts, Plants, 9, (2020)
[20] Santos I.L., Rodrigues A., Amante E.R., Silva L.H.M., Soursop (Annona muricata) Properties and Perspectives for Integral Valorization, Foods, 12, (2023)

← 1 2 3 4 5 6 →