Exosomes derived from adipose mesenchymal stem cells promote corneal injury repair and inhibit the formation of scars by anti-apoptosis

被引:0
作者
Ma, Chunli [1 ,2 ,3 ]
Li, Yixiao [2 ,4 ]
Liu, Baoling [5 ]
Deng, Junjie [1 ,2 ]
Gao, Xue [4 ,6 ]
Zhang, Huixin [3 ]
Zhang, Bingqiang [7 ]
Zhou, Qihui [3 ]
Peng, Xiaoting [3 ]
Zhang, Han [1 ,2 ]
机构
[1] Shandong First Med Univ, Shandong Prov Hosp, Jinan 250021, Peoples R China
[2] Shandong First Med Univ & Shandong Acad Med Sci, Jinan 271016, Peoples R China
[3] Univ Hlth & Rehabil Sci, Shandong Engn Res Ctr Tissue Rehabil Mat & Devices, Sch Rehabil Sci & Engn, Qingdao Key Lab Mat Tissue Repair & Rehabil, Qingdao 266113, Peoples R China
[4] Shandong Univ, Jinan 250100, Peoples R China
[5] Linyi Peoples Hosp, Dept Oncol, Linyi 276000, Peoples R China
[6] Shandong Univ, Dept Nephrol, Hosp 2, Jinan 250033, Peoples R China
[7] Qingdao Restore Med Testing Lab Co Ltd, Qingdao Key Lab Canc & Immune Cells, Qingdao 266111, Peoples R China
基金
中国国家自然科学基金;
关键词
Corneal injury repair; Exosomes; Mesenchymal stem cell; Anti-oxidant; Anti-apoptosis; NANOPARTICLES; REGENERATION; DISEASE;
D O I
10.1016/j.colsurfb.2024.114454
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In the corneal wound healing process, epithelial cell re-epithelialization and migration are the critical first steps following an injury. As the disease progresses, orderly regeneration of corneal stromal collagen and mild corneal stromal fibrosis are vital for corneal function reconstruction. Exosomes derived from adipose-derived mesenchymal stem cells (ADSCs-Exos) have emerged as a promising therapy due to their anti-oxidant, anti-apoptosis, and tissue repair properties. In this study, we successfully isolated exosomes via differential centrifugation and verified their effective extraction through transmission electron microscopy and nanoparticle tracking analysis. In vitro, ADSCs-Exos increased corneal epithelial cell migration by 20 % and reduced oxidative damage by 50 %. In addition, ADSCs-Exos demonstrated remarkable wound healing properties in corneal tissue. This effect was attributed to their ability to inhibit apoptosis of corneal stroma cells by upregulating Bax and downregulating Bcl2, reducing the Bax/Bcl2 protein expression ratio from 1 to 0.45. This decrease may subsequently inhibit alpha-SMA expression, thereby preventing corneal scarring. Overall, this research has elucidated the effects and potential targets of ADSCs-Exos in promoting corneal wound repair, offering a novel and promising approach for treating corneal injuries.
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页数:10
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