Integrated analysis of metabolome and transcriptome provides insights into the metabolic adjustments of heteroblastic foliage in Pinus massoniana seedlings

被引：0
作者：
Wang, Haoyun [1 ,2 ,3 ]
Wu, Feng [1 ,2 ,3 ]
Tu, Jingjing [3 ]
Liang, Daqu [3 ]
Zhao, Yuanxiang [3 ]
Ding, Guijie [1 ,2 ,3 ]
机构：
[1] Guizhou Univ, Inst Forest Resources & Environm Guizhou, Guiyang 550025, Peoples R China
[2] Guizhou Univ, Guizhou Key Lab Forest Cultivat Plateau Mt, Guiyang 550025, Peoples R China
[3] Guizhou Univ, Coll Forestry, Guiyang 550025, Peoples R China
来源：
INDUSTRIAL CROPS AND PRODUCTS | 2025年 / 226卷
基金：
中国国家自然科学基金;
关键词：
Metabolites; Comparative transcriptome; Flavonoid biosynthesis; Phytohormone; Primary and secondary needle; Conifer; ABSCISIC-ACID; BIOSYNTHESIS; EXPRESSION; PROTEINS; GROWTH;
D O I：
10.1016/j.indcrop.2025.120684
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Pine needle extract is rich in bioactive compounds. Pinus massoniana exhibits heteroblastic foliage in the first growing season, forming primary needle seedling (PNS) and secondary needle seedling (SNS). Significant differences in leaf morphology, photosynthetic physiology, and growth between PNS and SNS have been previously documented; however, the metabolic adjustments associated with heteroblastic foliage remain unclear. In this study, metabolomic and transcriptomic analyses revealed that elevated levels of auxin and cytokinin in PNS, mediated by high expression of IAA and ARR-A genes, hinder the germination and growth of axillary buds. In contrast, abscisic acid, jasmonic acid, and gibberellin acid levels increased in SNS, promoting the accumulation of phenylpropanoids, flavanones, and flavones. PNS also exhibited higher concentrations of amino acids and derivatives, along with flavonols, isoflavones, and anthocyanins, which could inhibit auxin polar transport and prevent auxin/cytokinin expulsion. Key genes such as PAL and CHS were up-regulated in SNS, while DFR, FLS, COMT, and PRDX6 were down-regulated, influencing flavonoid subclasses biosynthesis. Our findings demonstrate that phytohormones play a vital role in the metabolic adjustments of heteroblastic foliage through crosstalk between phytohormones and metabolites. These insights advance our understanding of the regulatory mechanisms and pharmaceutical potential associated with the heteroblastic foliage of conifers.
引用
收藏
页数：16
相关论文
共 77 条
  • [11] Climent J., Chambel M.R., Pardos M., Lario F., Villar-Salvador P., Biomass allocation and foliage heteroblasty in hard pine species respond differentially to reduction in rooting volume, Eur. J. For. Res., 130, pp. 841-850, (2011)
  • [12] Climent J., Dantas A.K., Alia R., Majada J., Clonal variation for shoot ontogenetic heteroblasty in maritime pine (Pinus pinaster Ait.), Trees, 27, pp. 1813-1819, (2013)
  • [13] Dinkeloo K., Boyd S., Pilot G., Update on amino acid transporter functions and on possible amino acid sensing mechanisms in plants, Semin. Cell Dev. Biol., 74, pp. 105-113, (2018)
  • [14] Dong X., Liu X., Cheng L., Li R., Ge S., Wang S., Cai Y., Liu Y., Meng S., Jiang C.Z., Shi C.L., Li T., Fu D., Qi M., Xu T., SlBEL11 regulates flavonoid biosynthesis, thus fine-tuning auxin efflux to prevent premature fruit drop in tomato, J. Integr. Plant Biol., 66, pp. 749-770, (2024)
  • [15] Duan H., Shao C., Luo X., Resco de Dios V., Tissue D.T., Ding G., Root relative water content is a potential signal for impending mortality of a subtropical conifer during extreme drought stress, Plant, Cell Environ., 46, pp. 2763-2777, (2023)
  • [16] Farquharson K.L., Examining the molecular basis of heterophylly in North American lake cress, Plant Cell, 26, pp. 45-67, (2014)
  • [17] Greenwood M.S., Day M.E., Berlyn G.P., Regulation of foliar plasticity in conifers: developmental and environmental factors, J. Sustain. For., 28, pp. 48-62, (2009)
  • [18] Guo J., Wu Y., Wang G., Wang T., Cao F., Integrated analysis of the transcriptome and metabolome in young and mature leaves of Ginkgo biloba L, Ind. Crops Prod., 143, (2020)
  • [19] Hausler R.E., Ludewig F., Krueger S., Amino acids–a life between metabolism and signaling, Plant Sci., 229, pp. 225-237, (2014)
  • [20] Hildreth S.B., Foley E.E., Muday G.K., Helm R.F., Winkel B.S., The dynamic response of the Arabidopsis root metabolome to auxin and ethylene is not predicted by changes in the transcriptome, Sci. Rep., 10, (2020)
12345678