Spatial transcriptome and single-cell RNA sequencing reveal the molecular basis of cotton fiber initiation development

被引：0
作者：
Zhang, Jun [1 ,2 ]
Chen, Rui [1 ]
Dai, Fan [1 ]
Tian, Yue [3 ]
Shi, Yue [1 ]
He, Ying [1 ]
Hu, Yan [1 ]
Zhang, Tianzhen [1 ]
机构：
[1] Zhejiang Univ, Adv Seed Inst, Plant Precis Breeding Acad, Coll Agr & Biotechnol,Zhejiang Prov Key Lab Crop G, Hangzhou, Peoples R China
[2] Zhejiang Acad Agr Sci, Inst Hort, Hangzhou, Zhejiang, Peoples R China
[3] Jiangsu Univ Sci & Technol, Coll Biotechnol, Zhenjiang, Peoples R China
来源：
PLANT JOURNAL | 2025年 / 121卷 / 06期
关键词：
Gossypium hirsutum L; single-cell transcriptomics; fiber differentiation; spatial transcriptome; SVBL; SVB; GENE-EXPRESSION; TRICHOME DEVELOPMENT; ARABIDOPSIS; ELONGATION; DIFFERENTIATION; FIDDLEHEAD; RESPONSES; PROFILES; ENCODES; WILD;
D O I：
10.1111/tpj.70064
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Recent advances in single-cell transcriptomics have greatly expanded our knowledge of plant development and cellular responses. However, analyzing fiber cell differentiation in plants, particularly in cotton, remains a complex challenge. A spatial transcriptomic map of ovule from -1 DPA, 0 DPA, and 1 DPA in cotton was successfully constructed, which helps to explain the important role of sucrose synthesis and lipid metabolism during early fiber development. Additionally, single-cell RNA sequencing (scRNA-seq) further highlighted the cellular heterogeneity and identified clusters of fiber developmental marker genes. Integration of spatial and scRNA-seq data unveiled key genes SVB and SVBL involved in fiber initiation, suggesting functional redundancy between them. These findings provide a detailed molecular landscape of cotton fiber development, offering valuable insights for enhancing lint yield.
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页数：15
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