Potentilla fulgens root extract rich in polyphenols ameliorate diabetic foot ulcers in wistar rats via regulating oxidative stress and connective tissue markers

Background: Potentilla fulgens (Wall.) ex Hook. (Rosaceae), commonly known as 'Bajradanti' is native to the lower Himalayan regions inclusive of the North-East India. Traditionally, the plant is used to treat chronic ailments like diabetes and diverse wounds, including gastric and mouth ulcers, and injuries from tiger bites. Objective: This study aims to assess the efficacy of P. fulgens root extract rich in polyphenolics in healing diabetic foot ulcer (DFU) in rats. Methods: DFU was induced in streptozotocin-nicotinamide diabetic rat feet by open excision wound model. Rats were divided into eight groups (n = 6/group): normal, negative control, plain gel (placebo) control, 1% silver sulfadiazine, and P. fulgens treated with topical ethyl acetate (EAPF, 5% and 10%) and methanol extract (MEPF, 5% and 10%). The morphological, histological and various biochemical parameters associated with the wound healing process such as connective tissue repair and oxidative stress biomarkers were evaluated. Results: Topical application of EAPF 10% and MEPF 10% over 21 days significantly (p < 0.05) reduced ulcer area in DFU rats compared to negative control. Furthermore, the extracts treated group notably (p < 0.05) increased levels of connective tissue biomarkers (total protein, hydroxyproline, hexosamine, and hexuronic acid) and antioxidant components (SOD and GSH), while significantly (p < 0.05) decreasing levels of lipid peroxidation (LPO) in foot tissue homogenate. Histological analysis of rats treated with the extracts revealed significant evidence of tissue repair, characterised by enhanced epithelial and collagen formation, along with decreased polymorphonuclear infiltration and edema. Conclusion:<bold> </bold>In summary, treatment with EAPF 10% significantly improved foot ulcers by modulating oxidative stress and connective tissue biomarkers in DFU animals.