Super-resolution imaging of proteins inside live mammalian cells with mLIVE-PAINT

被引：0

作者：

Bhaskar, Haresh ^{[1
,2
]}

Gidden, Zoe ^{[1
,3
]}

Virdi, Gurvir ^{[4
,5
,6
]}

Kleinjan, Dirk-Jan ^{[7
]}

Rosser, Susan J. ^{[7
]}

Gandhi, Sonia ^{[4
,5
,6
]}

Regan, Lynne ^{[1
,7
]}

Horrocks, Mathew H. ^{[2
,3
]}

机构：

[1] Univ Edinburgh, Sch Biol Sci, Edinburgh, Scotland

[2] Univ Edinburgh, Inst Regenerat & Repair, IRR Chem Hub, Edinburgh EH16 4UU, Scotland

[3] Univ Edinburgh, EaStCHEM Sch Chem, Edinburgh, Scotland

[4] Francis Crick Inst, 1 Midland Rd, London NW1 1AT, England

[5] UCL Queen Sq Inst Neurol, Dept Clin & Movement Neurosci, London, England

[6] Aligning Sci Parkinsons ASAP Collaborat Res Networ, Chevy Chase, MD USA

[7] Univ Edinburgh, Ctr Engn Biol, Sch Biol Sci, Edinburgh, Scotland

来源：

PROTEIN SCIENCE | 2025年 / 34卷 / 02期

基金：

英国惠康基金;

关键词：

dynamics; mitochondria; nucleus; peptide-peptide interactions; single-molecule; super-resolution microscopy; MICROSCOPY; FLUORESCENCE; EXPRESSION; RESOLUTION;

D O I：

10.1002/pro.70008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Super-resolution microscopy has revolutionized biological imaging, enabling the visualization of structures at the nanometer length scale. Its application in live cells, however, has remained challenging. To address this, we adapted LIVE-PAINT, an approach we established in yeast, for application in live mammalian cells. Using the 101A/101B coiled-coil peptide pair as a peptide-based targeting system, we successfully demonstrate the super-resolution imaging of two distinct proteins in mammalian cells, one localized in the nucleus, and the second in the cytoplasm. This study highlights the versatility of LIVE-PAINT, suggesting its potential for live-cell super-resolution imaging across a range of protein targets in mammalian cells. We name the mammalian cell version of our original method mLIVE-PAINT.

引用

页数：8

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