Green Analytical Chemistry Based Quality by Design Assisted UV Spectroscopic Method Development and Validation for Estimation of Chrysin

The objective of current research work to integrate Quality by Design (QbD) approach with principles of green analytical chemistry with a focus minimizing detrimental environmental effects. cost-efficient UV spectroscopic method was developed and validated as per ICH guidelines for chrysin analysis. Water was used as a major solvent. Sonication time scanning interval were chosen as independent variables and absorbance was selected as dependent variables. was used to optimize trial runs. To identify the optimized spectroscopic method, a simple 32 factorial design with factors and 2 levels was employed, resulting experimental runs. The optimized run was extensively validated as per ICH guidelines. A linear relationship determined between the concentrations ranging from 10 mu g/ml, with a regression coefficient of 0.998. precision assays showed a % RSD less than 2 indicating precise nature of the method. The percentage recovery ranged from 99.84 to 101.26% indicating promising accuracy. Sensitivity of the proposed method is proved its LOD and LOQ values which are 0.51 mu g/mL and mu g/mL respectively. The % RSD less than 2 indicates the developed method is rugged and robust. Additionally, the method exhibited promising repeatability. Hence, be concluded that integration of QbD and Green chemistry offers a promising avenue for developing a cost-effective and environment friendly analytical method for chrysin analysis.