Effects of Yersinia pseudotuberculosis outer membrane vesicles on Pseudomonas aeruginosa antigens immune response

被引：0
|
作者：
Duan, Zhongxu [1 ,2 ]
Song, Jingqi [1 ]
Zhang, Mingru [1 ]
Zhang, Zhe [1 ]
Li, Nan [3 ]
Fu, Yuqin [1 ]
Sun, Zhe [1 ]
Lu, Tiancheng [1 ]
Li, Siyuan [1 ]
Cao, Mingyue [1 ]
Wang, Qingyu [1 ]
Sun, Chunhui [4 ]
Wang, Xiuran [1 ,5 ,6 ]
机构：
[1] Jilin Agr Univ, Coll Life Sci, Engn Res Ctr Bioreactor & Drug Dev, Minist Educ, Changchun, Peoples R China
[2] Chinese Acad Sci, Northeast Inst Geog & Agroecol, State Key Lab Black Soils Conservat & Utilizat, Key Lab Wetland Ecol & Environm, Changchun, Peoples R China
[3] Chinese Acad Agr Sci, Changchun Vet Res Inst, Beijing, Peoples R China
[4] Sun Yat Sen Univ, Affiliated Hosp 7, Res Ctr, Shenzhen, Peoples R China
[5] Shandong First Med Univ & Shandong Acad Med Sci, Sch Lab Anim, Jinan, Peoples R China
[6] Shandong First Med Univ & Shandong Acad Med Sci, Shandong Lab Anim Ctr, Jinan, Peoples R China
来源：
PLOS ONE | 2024年 / 19卷 / 12期
关键词：
VACCINES; PROTEIN;
D O I：
10.1371/journal.pone.0310652
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Outer membrane vesicles (OMVs) are immunogenic self-adjuvanting vesicles produced by Gram-negative bacteria such as Pseudomonas aeruginosa and Yersinia pseudotuberculosis. While the effects of OMVs on different antigens immune stimulation are not clear. In this study, we constructed recombinant Yersinia pseudotuberculosis Delta lpxL strain,with pBlue-PcrV and pBlue-OprF/I, and then purified Delta lpxL rOMVPcrV (rOMVyp2P)and Delta lpxL rOMVOprF/I (rOMVyp2F) and analyzed its effect on immune response and protection against Pseudomonas aeruginosa PAO1 infection. The results showed that OMV assists in eliciting similar humoral immune responses to PcrV and OprF/I antigens. Delta lpxL rOMVPcrV and Delta lpxL rOMVOprF/I elicited Th1/Th2 balanced immune response, and higher IgM and IgA antibodies.However, there are differences in immune protection for the pulmonary. The survival rate of mice in Delta lpxL rOMVPcrV group was 20%, which was significantly better than that in Delta lpxL rOMVOprF/I group. Delta lpxL OMVPcrV is better cooperation for Pseudomonas immune protection in lung.
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页数：16
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