Immunological characteristics of bronchoalveolar lavage fluid and blood across connective tissue disease-associated interstitial lung diseases

被引：2

作者：

Hirano, Aiko ^{[1
]}

Sakashita, Aki ^{[1
]}

Fujii, Wataru ^{[1
]}

Bassler, Kevin ^{[2
]}

Tsuji, Taisuke ^{[3
]}

Kadoya, Masatoshi ^{[4
]}

Omoto, Atsushi ^{[4
]}

Hiraoka, Noriya ^{[3
]}

Imabayashi, Tatsuya ^{[5
]}

Kaneko, Yoshiko ^{[5
]}

Sofue, Hideaki ^{[1
]}

Maehara, Yosuke ^{[6
]}

Seno, Takahiro ^{[1
]}

Wada, Makoto ^{[1
]}

Kohno, Masataka ^{[1
]}

Fukuda, Wataru ^{[4
]}

Yamada, Kei ^{[6
]}

Takayama, Koichi ^{[5
]}

Kawahito, Yutaka ^{[1
]}

机构：

[1] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Inflammat & Immunol, Kyoto, Japan

[2] Aimed Analyt GmbH, Bonn, Germany

[3] Japanese Red Cross Kyoto Daiichi Hosp, Dept Resp Med, Kyoto, Japan

[4] Japanese Red Cross Kyoto Daiichi Hosp, Ctr Rheumat Dis, Kyoto, Japan

[5] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Pulm Med, Kyoto, Japan

[6] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Radiol, Kyoto, Japan

来源：

FRONTIERS IN IMMUNOLOGY | 2024年 / 15卷

关键词：

single-cell RNA sequencing; genetics; interstitial lung disease; connective tissue disease; systemic autoimmune rheumatic disease; RHEUMATOLOGY CLASSIFICATION CRITERIA; 2022; AMERICAN-COLLEGE; ENDOPLASMIC-RETICULUM STRESS; SJOGRENS-SYNDROME; SYSTEMIC-SCLEROSIS; CELLS; LEAGUE; ALLIANCE; GRANULOMATOSIS; PATHOGENESIS;

D O I：

10.3389/fimmu.2024.1408880

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Interstitial lung disease (ILD) is a serious complication of connective tissue diseases (CTDs). The heterogeneity of ILDs reflects differences in pathogenesis among diseases. This study aimed to clarify the characteristics of CTD-ILDs via a detailed analysis of the bronchoalveolar lavage fluid (BALF) and blood immune cells. BALF and blood samples were collected from 39 Japanese patients with newly diagnosed ILD: five patients with Sj & ouml;gren's syndrome (SS), eight patients with dermatomyositis (DM), six patients with rheumatoid arthritis (RA), six patients with systemic sclerosis, four patients with anti-neutrophil cytoplasmic antibody-associated vasculitis, and 10 patients with idiopathic interstitial pneumonia. We performed single-cell RNA sequencing to analyze the gene expression profiles in these patients' immune cells. In patients with SS, B cells in the BALF were increased and genes associated with the innate and acquired immunity were enriched in both the BALF and blood. In contrast, patients with DM showed an upregulation of genes associated with viral infection in both the BALF and blood. In patients with RA, neutrophils in the BALF tended to increase, and their gene expression patterns changed towards inflammation. These disease-specific characteristics may help us understand the pathogenesis for each disease and discover potential biomarkers.

引用

页数：19

共 60 条

[1] Pathogenesis, clinical features, and treatment strategy for rheumatoid arthritis-associated interstitial lung disease [J].

Akiyama, Mitsuhiro ;

Kaneko, Yuko .

AUTOIMMUNITY REVIEWS, 2022, 21 (05)

[2] Pivotal clinical dilemmas in collagen vascular diseases associated with interstitial lung involvement [J].

Antoniou, K. M. ;

Margaritopoulos, G. ;

Economidou, F. ;

Siafakas, N. M. .

EUROPEAN RESPIRATORY JOURNAL, 2009, 33 (04) :882-896

[3] C1q enhances IFN-γ production by antigen-specific T cells via the CD40 costimulatory pathway on dendritic cells [J].

Baruah, Paramita ;

Dumitriu, Ingrid E. ;

Malik, Talat H. ;

Cook, H. Terence ;

Dyson, Julian ;

Scott, Diane ;

Simpson, Elizabeth ;

Botto, Marina .

BLOOD, 2009, 113 (15) :3485-3493

[4] Alveolar macrophages in early stage COPD show functional deviations with properties of impaired immune activation [J].

Bassler, Kevin ;

Fujii, Wataru ;

Kapellos, Theodore S. ;

Dudkin, Erika ;

Reusch, Nico ;

Horne, Ari ;

Reiz, Benedikt ;

Luecken, Malte D. ;

Osei-Sarpong, Collins ;

Warnat-Herresthal, Stefanie ;

Bonaguro, Lorenzo ;

Schulte-Schrepping, Jonas ;

Wagner, Allon ;

Guenther, Patrick ;

Pizarro, Carmen ;

Schreiber, Tina ;

Knoll, Rainer ;

Holsten, Lisa ;

Kroeger, Charlotte ;

De Domenico, Elena ;

Becker, Matthias ;

Haendler, Kristian ;

Wohnhaas, Christian T. ;

Baumgartner, Florian ;

Koehler, Meike ;

Theis, Heidi ;

Kraut, Michael ;

Wadsworth, Marc H. ;

Hughes, Travis K. ;

Ferreira, Humberto J. ;

Hinkley, Emily ;

Kaltheuner, Ines H. ;

Geyer, Matthias ;

Thiele, Christoph ;

Shalek, Alex K. ;

Feisst, Andreas ;

Thomas, Daniel ;

Dickten, Henning ;

Beyer, Marc ;

Baum, Patrick ;

Yosef, Nir ;

Aschenbrenner, Anna C. ;

Ulas, Thomas ;

Hasenauer, Jan ;

Theis, Fabian J. ;

Skowasch, Dirk ;

Schultze, Joachim L. .

FRONTIERS IN IMMUNOLOGY, 2022, 13

[5] Prevalence of interferon type I signature in CD14 monocytes of patients with Sjogren's syndrome and association with disease activity and BAFF gene expression [J].

Brkic, Zana ;

Maria, Naomi I. ;

van Helden-Meeuwsen, Cornelia G. ;

van de Merwe, Joop P. ;

van Daele, Paul L. ;

Dalm, Virgil A. ;

Wildenberg, Manon E. ;

Beumer, Wouter ;

Drexhage, Hemmo A. ;

Versnel, Marjan A. .

ANNALS OF THE RHEUMATIC DISEASES, 2013, 72 (05) :728-735

[6] Tobacco smoke exposure recruits inflammatory airspace monocytes that establish permissive lung niches for Mycobacterium tuberculosis [J].

Corleis, Bjoern ;

Tzouanas, Constantine N. ;

Wadsworth II, Marc H. ;

Cho, Josalyn L. ;

Linder, Alice H. ;

Schiff, Abigail E. ;

Zessin, Bjoern ;

Stei, Fabian ;

Dorhoi, Anca ;

Dickey, Amy K. ;

Medoff, Benjamin D. ;

Shalek, Alex K. ;

Kwon, Douglas S. .

SCIENCE TRANSLATIONAL MEDICINE, 2023, 15 (725)

[7] Lymphocytic alveolitis: a surprising index of poor prognosis in patients with primary Sjogren's syndrome [J].

Dalavanga, Y. A. ;

Voulgari, P. V. ;

Georgiadis, A. N. ;

Leontaridi, C. ;

Katsenos, S. ;

Vassiliou, M. ;

Drosos, A. A. ;

Constantopoulos, S. H. .

RHEUMATOLOGY INTERNATIONAL, 2006, 26 (09) :799-804

[8] Predictors of progression in systemic sclerosis patients with interstitial lung disease [J].

Distler, Oliver ;

Assassi, Shervin ;

Cottin, Vincent ;

Cutolo, Maurizio ;

Danoff, Sonye K. ;

Denton, Christopher P. ;

Distler, Jorg H. W. ;

Hoffmann-Vold, Anna-Maria ;

Johnson, Sindhu R. ;

Ladner, Ulf Mueller ;

Smith, Vanessa ;

Volkmann, Elizabeth R. ;

Maher, Toby M. .

EUROPEAN RESPIRATORY JOURNAL, 2020, 55 (05)

[9] Alveolar macrophage transcriptomic profiling in COPD shows major lipid metabolism changes [J].

Fujii, Wataru ;

Kapellos, Theodore S. ;

Bassler, Kevin ;

Haendler, Kristian ;

Holsten, Lisa ;

Knoll, Rainer ;

Warnat-Herresthal, Stefanie ;

Oestreich, Marie ;

Hinkley, Emily R. ;

Hasenauer, Jan ;

Pizarro, Carmen ;

Thiele, Christoph ;

Aschenbrenner, Anna C. ;

Ulas, Thomas ;

Skowasch, Dirk ;

Schultze, Joachim L. .

ERJ OPEN RESEARCH, 2021, 7 (03)

[10] Osteopontin Links Myeloid Activation and Disease Progression in Systemic Sclerosis [J].

Gao, Xia ;

Jia, Guiquan ;

Guttman, Anna ;

DePianto, Daryle J. ;

Morshead, Katrina B. ;

Sun, Kai-Hui ;

Ramamoorthi, Nandhini ;

Vander Heiden, Jason A. ;

Modrusan, Zora ;

Wolters, Paul J. ;

Jahreis, Angelika ;

Arron, Joseph R. ;

Khanna, Dinesh ;

Ramalingam, Thirumalai R. .

CELL REPORTS MEDICINE, 2020, 1 (08)

← 1 2 3 4 5 6 →