RNA Interference Applied to Crustacean Aquaculture

被引:2
|
作者
Fajardo, Carlos [1 ,2 ]
De Donato, Marcos [3 ,4 ]
Macedo, Marta [2 ,5 ]
Charoonnart, Patai [6 ,7 ]
Saksmerprome, Vanvimon [6 ,7 ]
Yang, Luyao [8 ]
Purton, Saul [8 ]
Mancera, Juan Miguel [1 ]
Costas, Benjamin [2 ,5 ]
机构
[1] Univ Cadiz, Inst Univ Invest Marina INMAR, Fac Marine & Environm Sci, Dept Biol, Campus Excelencia Int Mar CEI MAR, Cadiz 11510, Spain
[2] Univ Porto CIIMAR, Interdisciplinary Ctr Marine & Environm Res, P-4450208 Matosinhos, Portugal
[3] Ctr Aquaculture Technol CAT, San Diego, CA 92121 USA
[4] Escuela Med & Ciencias Salud Tecnol Monterrey, Monterrey 76130, Mexico
[5] Univ Porto UP, Inst Biomed Sci Abel Salazar, ICBAS, P-4050313 Porto, Portugal
[6] Mahidol Univ, Fac Sci, CENTEX SHRIMP, Ctr Excellence Shrimp Mol Biol & Biotechnol, Bangkok 10400, Thailand
[7] Natl Sci & Technol Dev Agcy NSTDA, Natl Ctr Genet Engn & Biotechnol BIOTEC, Bangkok 12120, Thailand
[8] Univ Coll London UCL, Dept Struct & Mol Biol, London WC1E 6BT, England
关键词
anti-viral; dsRNA; gene-silencing; immunology; innate immunity; miRNA; nanoencapsulation; RNAi; shrimp; siRNA; DOUBLE-STRANDED-RNA; SPOT-SYNDROME-VIRUS; MACROBRACHIUM-ROSENBERGII NODAVIRUS; YELLOW HEAD VIRUS; BLACK TIGER SHRIMP; PENAEUS-STYLIROSTRIS DENSOVIRUS; INFECTIOUS MYONECROSIS VIRUS; PACIFIC WHITE SHRIMP; TAURA SYNDROME VIRUS; LITOPENAEUS-VANNAMEI;
D O I
10.3390/biom14111358
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
RNA interference (RNAi) is a powerful tool that can be used to specifically knock-down gene expression using double-stranded RNA (dsRNA) effector molecules. This approach can be used in aquaculture as an investigation instrument and to improve the immune responses against viral pathogens, among other applications. Although this method was first described in shrimp in the mid-2000s, at present, no practical approach has been developed for the use of dsRNA in shrimp farms, as the limiting factor for farm-scale usage in the aquaculture sector is the lack of cost-effective and simple dsRNA synthesis and administration procedures. Despite these limitations, different RNAi-based approaches have been successfully tested at the laboratory level, with a particular focus on shrimp. The use of RNAi technology is particularly attractive for the shrimp industry because crustaceans do not have an adaptive immune system, making traditional vaccination methods unfeasible. This review summarizes recent studies and the state-of-the-art on the mechanism of action, design, use, and administration methods of dsRNA, as applied to shrimp. In addition, potential constraints that may hinder the deployment of RNAi-based methods in the crustacean aquaculture sector are considered.
引用
收藏
页数:38
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